PMID: 3384090Jun 6, 1988Paper

Crystallization of cytochrome P-450scc from bovine adrenocortical mitochondria

FEBS Letters
Y IwamotoY Ichikawa

Abstract

Cytochrome P-450scc (P-450scc), a cholesterol side-chain cleavage enzyme from bovine adrenocortical mitochondria, has been crystallized for the first time. Upon removal of glycerol from the solution of the native enzyme complexed with pyridoxal 5'-phosphate (PLP) by microdialysis against distilled water, reddish and planar crystals appeared. The crystals of native P-450scc were also obtained by the same procedure. We identified the crystals as the P-450scc-PLP complex or native P-450scc by absorption spectroscopy and SDS-polyacrylamide gel electrophoresis, and characterized them under a polarization microscope.

References

Jun 5, 1987·Journal of Molecular Biology·T L PoulosA J Howard
Apr 1, 1967·Journal of Biochemistry·T Yoro, S Horie
Jul 6, 1966·Biochemical and Biophysical Research Communications·E R Simpson, G S Boyd
Oct 23, 1967·Biochimica Et Biophysica Acta·A C Appleby
Oct 1, 1967·European Journal of Biochemistry·E R Simpson, G S Boyd
May 18, 1965·Biochimica Et Biophysica Acta·L D WILSONB W HARDING

❮ Previous
Next ❯

Citations

Jan 1, 1990·Critical Reviews in Biochemistry and Molecular Biology·F P Guengerich
Oct 1, 1989·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·G G Gibson
Nov 1, 1993·Pharmazie in unserer Zeit·K Ruckpaul
May 31, 1990·European Journal of Biochemistry·M Degli EspostiG Venturoli
Jan 1, 1996·Biochimie·Y T ChangG H Loew
Dec 1, 1995·Toxicology Letters·M R WatermanI Pikuleva
Dec 28, 1992·Biochimica Et Biophysica Acta·S Vijayakumar, J C Salerno
Jan 1, 1991·The International Journal of Biochemistry·K IwahashiY Ichikawa
Apr 6, 2005·Journal of Theoretical Biology·Victor Sivozhelezov, Claudio Nicolini

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.