Crystallization of oxytetracycline from fermentation waste liquor: influence of biopolymer impurities

Journal of Colloid and Interface Science
Shi-zhong LiDianzuo Wang

Abstract

Organic impurities in the fermentation broth of antibiotic production impose great difficulties in the crystallization and recovery of antibiotics from the concentrated waste liquor. In the present laboratory study, the inhibitory effect of biopolymers on antibiotic crystallization was investigated using oxytetracycline (OTC) as the model antibiotic. Organic impurities separated from actual OTC fermentation waste liquor by ultrafiltration were dosed into a pure OTC solution at various concentrations. The results demonstrated that small organic molecules with an apparent molecular weight (AMW) of below 10,000 Da did not affect OTC crystallization significantly. However, large biopolymers, especially polysaccharides, in the fermentation waste caused severe retardation of crystal growth and considerable deterioration in the purity of the OTC crystallized. Atomic force microscopy (AFM) revealed that OTC nuclei formed in the solution attached to the surfaces of large organic molecules, probably polysaccharides, instead of being surrounded by proteins as previously thought. It is proposed that the attachment of OTC nuclei to biopolymers would prevent OTC from rapid crystallization, resulting in a high OTC residue in the aqueous phase...Continue Reading

References

Apr 1, 1999·Biotechnology and Bioengineering·R A JudgeM L Pusey
Aug 15, 2000·Annual Review of Biophysics and Biomolecular Structure·A McPherson Kuznetsov YuG
Feb 13, 2001·International Journal of Pharmaceutics·S L RaghavanJ Hadgraft
Jul 27, 2001·Acta Crystallographica. Section D, Biological Crystallography·A McPhersonM Plomp
Apr 30, 2003·Journal of Structural Biology·Alexander McPhersonMarco Plomp
Nov 1, 2005·Journal of Colloid and Interface Science·Xiao-Yan Li, Jian-Jun Zhang

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Citations

Apr 12, 2006·Applied Microbiology and Biotechnology·Evelyn M Buque-TaboadaLuuk A M van der Wielen

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