Crystallization of phycoerythrin 545 of Rhodomonas lens using detergents and unusual additives

Protein Science : a Publication of the Protein Society
M BeckerR Huber

Abstract

Phycoerythrin 545 from the cryptomonad alga, Rhodomonas lens, has been crystallized under a wide variety of conditions. Although this type of photosynthetic light-harvesting protein is water soluble, detergents were always required for crystallization. The crystals were typically poorly ordered, or ordered in only two dimensions. However, crystals that were well-ordered in three dimensions could be obtained under two different conditions. Both used polyethylene glycol as precipitant and the detergent lauryldimethylaminoxide, but the additives that were critical for obtaining well-ordered crystals were propionamide in one case and Cs+/Br- in the other. Crystals obtained in the presence of propionamide have the space group P2(1)2(1)2(1), with cell constants of a = 85.6 angstroms, b = 108.2 angstroms, and c = 131.0 angstroms, and contain two dimers [i.e., 2 x (alpha2beta2)] in the asymmetric unit. They show diffraction to at least 3.0 angstroms resolution. The crystals grown with Cs+/Br- are nearly isomorphous. Both types of crystals show intense, strongly polarized fluorescence, suggesting that energy transfer in the crystals is highly efficient. This should provide a basis for quantitative investigation of the role of exciton in...Continue Reading

References

Jul 20, 1989·Nature·J M Enoch, V Lakshminarayanan
Nov 14, 1969·Journal of Molecular Biology·P A EaglesH Gutfreund
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
May 31, 1994·Biochemistry·R MacCollP McNaughton
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Nov 1, 1995·Photosynthesis Research·A N Glazer, G J Wedemayer

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