Crystallization of the C-terminal domain of the addiction antidote CcdA in complex with its toxin CcdB

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Lieven ButsMinh-Hoa Dao-Thi

Abstract

CcdA and CcdB are the antidote and toxin of the ccd addiction module of Escherichia coli plasmid F. The CcdA C-terminal domain (CcdAC36; 36 amino acids) was crystallized in complex with CcdB (dimer of 2 x 101 amino acids) in three different crystal forms, two of which diffract to high resolution. Form II belongs to space group P2(1)2(1)2(1), with unit-cell parameters a = 37.6, b = 60.5, c = 83.8 A and diffracts to 1.8 A resolution. Form III belongs to space group P2(1), with unit-cell parameters a = 41.0, b = 37.9, c = 69.6 A, beta = 96.9 degrees, and diffracts to 1.9 A resolution.

References

Aug 5, 1992·Journal of Molecular Biology·P Bernard, M Couturier
May 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·K GerdesS Molin
Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Sep 1, 1994·Molecular & General Genetics : MGG·M A SalmonM Couturier
Jan 1, 1993·Annual Review of Biochemistry·B W Matthews
Jun 11, 1996·Proceedings of the National Academy of Sciences of the United States of America·E AizenmanG Glaser
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·M H Dao-ThiR Loris
Jan 26, 1999·Journal of Molecular Biology·R LorisL Wyns
Nov 5, 1999·Annual Review of Microbiology·H Engelberg-Kulka, G Glaser
Jun 30, 2000·Journal of Molecular Biology·M H Dao-ThiJ Backmann
Aug 22, 2001·Biochemistry·S De VosR Loris
Nov 22, 2001·Proceedings of the National Academy of Sciences of the United States of America·S K ChristensenK Gerdes
Dec 14, 2001·The Journal of Biological Chemistry·Minh-Hoa Dao-ThiJan Backmann
Apr 30, 2003·Molecular Cell·Katsuhiko KamadaStephen K Burley
May 14, 2003·The Journal of Biological Chemistry·Remy LorisLode Wyns
Jul 26, 2003·Science·Hanna Engelberg-Kulka, Ronen Hazan
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Katherine A Kantardjieff, Bernhard Rupp
Mar 24, 2004·Trends in Microbiology·Hanna Engelberg-KulkaRonen Hazan
Dec 4, 2004·Journal of Bacteriology·Shahar AmitaiHanna Engelberg-Kulka
Feb 19, 2005·Nucleic Acids Research·Deo Prakash Pandey, Kenn Gerdes
Apr 28, 2005·Journal of Molecular Biology·Minh-Hoa Dao-ThiRemy Loris
May 3, 2005·Nature Reviews. Microbiology·Kenn GerdesAnders Løbner-Olesen

❮ Previous
Next ❯

Citations

May 26, 2009·The Journal of Biological Chemistry·Mario SimicJurij Lah
Aug 4, 2009·Molecular Cell·Natalie De JongeRemy Loris
Aug 29, 2009·Journal of Molecular Biology·Djordje Francuski, Wolfram Saenger
Jan 8, 2013·Journal of the American Chemical Society·Igor DrobnakJurij Lah

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Crystallographica. Section D, Biological Crystallography
Minh Hoa Dao-ThiRemy Loris
Nature Reviews. Microbiology
K GerdesAnders Løbner-Olesen
Journal of Molecular Biology
Minh-Hoa Dao-ThiRemy Loris
© 2021 Meta ULC. All rights reserved