Crystallization of the C-terminal globular domain of avian reovirus fibre

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Mark J van RaaijJavier Benavente

Abstract

Avian reovirus fibre, a homotrimer of the sigmaC protein, is responsible for primary host-cell attachment. Using the protease trypsin, a C-terminal sigmaC fragment containing amino acids 156-326 has been generated which was subsequently purified and crystallized. Two different crystal forms were obtained, one grown in the absence of divalent cations and belonging to space group P6(3)22 (unit-cell parameters a = 75.6, c = 243.1 A) and one grown in the presence of either zinc or cadmium sulfate and belonging to space group P321 (unit-cell parameters a = 74.7, c = 74.5 A and a = 73.1, c = 69.9 A for the Zn(II)- and Cd(II)-grown crystals, respectively). The first crystal form diffracted synchrotron radiation to 3.0 A resolution and the second form to 2.2-2.3 A. Its closest related structure, the C-terminal fragment of mammalian reovirus fibre, has only 18% sequence identity and molecular-replacement attempts were unsuccessful. Therefore, a search is under way for suitable heavy-atom derivatives and attempts are being made to grow protein crystals containing selenomethionine instead of methionine.

References

Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·H R Powell
Oct 26, 1999·Acta Crystallographica. Section D, Biological Crystallography·A G Leslie
Mar 10, 2001·Cell·E S BartonT S Dermody

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Citations

May 10, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Pablo Guardado CalvoMark J van Raaij
Feb 3, 2012·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Carmela Garcia-Doval, Mark J van Raaij
Oct 21, 2005·Journal of Molecular Biology·Pablo Guardado CalvoMark J van Raaij
Feb 17, 2009·The Journal of General Virology·Pablo Guardado-CalvoMark J van Raaij

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