Crystallization of the glycogen-binding domain of the AMP-activated protein kinase beta subunit and preliminary X-ray analysis

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Galina PolekhinaMichael W Parker

Abstract

AMP-activated protein kinase (AMPK) is an intracellular energy sensor that regulates metabolism in response to energy demand and supply by adjusting the ATP-generating and ATP-consuming pathways. AMPK potentially plays a critical role in diabetes and obesity as it is known to be activated by metforin and rosiglitazone, drugs used for the treatment of type II diabetes. AMPK is a heterotrimer composed of a catalytic alpha subunit and two regulatory subunits, beta and gamma. Mutations in the gamma subunit are known to cause glycogen accumulation, leading to cardiac arrhythmias. Recently, a functional glycogen-binding domain (GBD) has been identified in the beta subunit. Here, the crystallization of GBD in the presence of beta-cyclodextrin is reported together with preliminary X-ray data analysis allowing the determination of the structure by single isomorphous replacement and threefold averaging using in-house X-ray data collected from a selenomethionine-substituted protein.

References

Apr 28, 1968·Journal of Molecular Biology·B W Matthews
Mar 24, 1999·Trends in Biochemical Sciences·B E KempL A Witters
May 17, 2002·Current Opinion in Cardiology·Michael H GollobRobert Roberts
May 16, 2003·Current Biology : CB·Galina PolekhinaDavid Stapleton
Jun 28, 2003·FEBS Letters·D Grahame HardieEmma R Hudson
Aug 22, 2003·Protein Science : a Publication of the Protein Society·Nikos PinotsisIrene M Mavridis
Nov 15, 2003·Current Biology : CB·Angela WoodsDavid Carling
Jan 15, 2004·The Journal of Clinical Investigation·John W ScottD Grahame Hardie
Jan 20, 2004·Trends in Biochemical Sciences·David Carling

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