Crystallization of the receptor binding domain of vascular endothelial growth factor

Proteins
H W ChristingerAbraham M de Vos

Abstract

Vascular endothelial growth factor (VEGF) is a potent angiogenic factor with a unique specificity for vascular endothelial cells. In addition to its role in vasculogenesis and embryonic angiogenesis, VEGF is implicated in pathologic neovascularization associated with tumors and diabetic retinopathy. Four different constructs of a short variant of VEGF sufficient for receptor binding were overexpressed in Escherichia coli, refolded, purified, and crystallized in five different space groups. In order to facilitate the production of heavy atom derivatives, single cysteine mutants were designed based on the crystal structure of platelet-derived growth factor. A construct consisting of residues 8 to 109 was crystallized in space group P2(1), with cell parameters a = 55.6 A, b = 60.4 A, c = 77.7 A, beta = 90.0 degrees, and four monomers in the asymmetric unit. Native and derivative data were collected for two of the cysteine mutants as well as for wild-type VEGF.

References

Jan 1, 1994·Progress in Growth Factor Research·G NeufeldB Z Levi
Jun 1, 1995·Current Opinion in Structural Biology·N W Isaacs
Jan 1, 1995·Annual Review of Biophysics and Biomolecular Structure·P D Sun, D R Davies
Jun 9, 1994·Nature·A J LapthornN W Isaacs
Oct 1, 1993·Proteins·S DaopinD R Davies
May 20, 1993·Journal of Molecular Biology·M P Schlunegger, M G Grütter
Jan 1, 1995·Breast Cancer Research and Treatment·N Ferrara

❮ Previous
Next ❯

Citations

Apr 5, 2007·Proceedings of the National Academy of Sciences of the United States of America·Craig W Vander KooiDaniel J Leahy
Jul 8, 1997·Proceedings of the National Academy of Sciences of the United States of America·Y A MullerA M de Vos
Oct 23, 1997·Protein Science : a Publication of the Protein Society·W J FairbrotherM A Starovasnik
Feb 9, 2006·Cellular and Molecular Life Sciences : CMLS·S Cébe-SuarezK Ballmer-Hofer
Feb 8, 2000·Biological Chemistry·P ScheideggerR Jaussi
Aug 14, 2009·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Bridget F MurphyMichael B Thompson
Jul 20, 2002·Archives of Biochemistry and Biophysics·Nicholas J GasparUte Schellenberger
Apr 17, 2008·Analytical Biochemistry·Kimberly J PetersonSamuel H Gellman
Mar 10, 2015·International Journal of Biological Macromolecules·S Shirin ShahangianReyhaneh Sariri
Dec 2, 1999·Experimental Cell Research·T V PetrovaK Alitalo
Aug 7, 2002·The Journal of Pharmacy and Pharmacology·Tae-Kyoung Kim, Diane J Burgess
Aug 2, 2011·Chemistry & Biology·Anna FedorovaKurt Deshayes
Aug 16, 2016·Carbohydrate Polymers·Michael S RiedererMelissa D Krebs
Nov 3, 2016·Proceedings of the National Academy of Sciences of the United States of America·Madelon Q WentinkPeter Timmerman
Nov 2, 1999·Journal of Molecular Biology·M A StarovasnikN J Skelton
Sep 3, 2020·Analytical and Bioanalytical Chemistry·Zahra S RezaeiReza H Sajedi
Jun 16, 2010·Langmuir : the ACS Journal of Surfaces and Colloids·Ana SalaFranz E Weber
Feb 28, 2002·Journal of Molecular Biology·Borlan PanWayne J Fairbrother

❮ Previous
Next ❯

Related Concepts

Related Feeds

Arterial-Venous in Development & Disease

Arterial-venous development may play a crucial role in cardiovascular diseases. Here is the latest research.