Crystallization of the SH2-binding site of p130Cas in complex with Lck, a Src-family kinase

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Fariborz NasertorabiKathryn R Ely

Abstract

Cas-family proteins serve as docking proteins in integrin-mediated signal transduction. The founding member of this family, p130Cas, becomes tyrosine-phosphorylated in response to extracellular stimuli such as integrin-mediated cell adhesion and ligand engagement of receptor tyrosine kinases. Cas proteins are large multidomain molecules that transmit signals as intermediaries through interactions with signaling molecules such as FAK and other tyrosine kinases, as well as tyrosine phosphatases. After Cas is tyrosine-phosphorylated, it acts as a docking protein for binding SH2 domains of Src-family kinases. In order to examine the structural basis for a key step in propagation of signals by Cas, one of the major SH2-binding sites of Cas has been crystallized in complex with the SH3-SH2 regulatory domains of the Src-family kinase Lck. Crystallization conditions were identified by high-throughput screening and optimized with multiple rounds of seeding. The crystals formed at 295 K in space group P2(1)2(1)2(1), with unit-cell parameters a = 77.4, b = 107.3, c = 166.4 A, and diffract to 2.7 A resolution.

References

Nov 7, 1995·Proceedings of the National Academy of Sciences of the United States of America·T R Polte, S K Hanks
Apr 12, 1996·The Journal of Biological Chemistry·T NakamotoH Hirai
Aug 14, 1999·Science·F G Giancotti, E Ruoslahti
Feb 17, 2000·Trends in Cell Biology·G M O'NeillE A Golemis
Apr 30, 2003·Journal of Structural Biology·Joseph R LuftGeorge T DeTitta

❮ Previous
Next ❯

Citations

Oct 1, 2011·Journal of the National Cancer Institute·Alpa M NickAnil K Sood
Feb 12, 2013·Chemical Society Reviews·Dziyana KraskouskayaPatrick T Gunning

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Related Papers

Biochemical and Biophysical Research Communications
Fariborz NasertorabiKathryn R Ely
Journal of Molecular Recognition : JMR
Fariborz NasertorabiKathryn R Ely
© 2021 Meta ULC. All rights reserved