Crystallization, X-ray diffraction analysis and phasing of 17beta-hydroxysteroid dehydrogenase from the fungus Cochliobolus lunatus

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Alberto CassettaDoriano Lamba

Abstract

17beta-Hydroxysteroid dehydrogenase from the filamentous fungus Cochliobolus lunatus (17beta-HSDcl) is an NADP(H)-dependent enzyme that preferentially catalyses the oxidoreduction of oestrogens and androgens. The enzyme belongs to the short-chain dehydrogenase/reductase superfamily and is the only fungal hydroxysteroid dehydrogenase known to date. 17beta-HSDcl has recently been characterized and cloned and has been the subject of several functional studies. Although several hypotheses on the physiological role of 17beta-HSDcl in fungal metabolism have been formulated, its function is still unclear. An X-ray crystallographic study has been undertaken and the optimal conditions for crystallization of 17beta-HSDcl (apo form) were established, resulting in well shaped crystals that diffracted to 1.7 A resolution. The space group was identified as I4(1)22, with unit-cell parameters a = b = 67.14, c = 266.77 A. Phasing was successfully performed by Patterson search techniques. A catalytic inactive mutant Tyr167Phe was also engineered, expressed, purified and crystallized for functional and structural studies.

References

Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jun 1, 1997·Endocrine Reviews·T M Penning
May 23, 2001·Archives of Biochemistry and Biophysics·T L RiznerJ Stojan
Sep 22, 2001·Acta Crystallographica. Section D, Biological Crystallography·J Navaza
Sep 17, 2002·European Journal of Biochemistry·Yvonne KallbergBengt Persson
Apr 30, 2003·Canadian Journal of Microbiology·Tea Lanisnik Rizner, Michael H Wheeler
Jul 17, 2003·Human Reproduction Update·Trevor M Penning
May 8, 2004·Molecular and Cellular Endocrinology·R MindnichJ Adamski

❮ Previous
Next ❯

Citations

May 9, 2012·Applied Microbiology and Biotechnology·Marina V Donova, Olga V Egorova
Sep 29, 2011·The Journal of Steroid Biochemistry and Molecular Biology·Katja Kristan, Tea Lanišnik Rižner
Jan 2, 2007·Journal of Biotechnology·Katja KristanTea Lanisnik Rizner
Aug 29, 2006·The FEBS Journal·Natasa Poklar Ulrih, Tea Lanisnik Rizner
May 25, 2016·Neuroscience and Biobehavioral Reviews·Ian P Burges WatsonAdrian J Bradley
Jan 30, 2020·Applied Microbiology and Biotechnology·Amit PratushZhong Hu
Nov 20, 2016·Microbial Biotechnology·Lorena Fernández-CabezónJosé L García
Jun 15, 2011·Journal of Chemical Information and Modeling·Mojca Brunskole SvegeljStanislav Gobec

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.