PMID: 9533628Apr 9, 1998Paper

Crystallographic and spectroscopic characterization of a molecular hinge: conformational changes in bothropstoxin I, a dimeric Lys49-phospholipase A2 homologue

Proteins
M T da Silva GiottoRichard J Ward

Abstract

Bothropstoxin I (BthTX-I) from the venom of Bothrops jararacussu is a myotoxic phospholipase A2 (PLA2) homologue which, although catalytically inactive due to an Asp49-->Lys substitution, disrupts the integrity of lipid membranes by a Ca2+-independent mechanism. The crystal structures of two dimeric forms of BthTX-I which diffract X-rays to resolutions of 3.1 and 2.1 angstroms have been determined. The monomers in both structures are related by an almost perfect twofold axis of rotation and the dimer interfaces are defined by contacts between the N-terminal alpha-helical regions and the tips of the beta-wings of partner monomers. Significant differences in the relative orientation of the monomers in the two crystal forms results in "open" and "closed" dimer conformations. Spectroscopic investigations of BthTX-I in solution have correlated these conformational differences with changes in the intrinsic fluorescence emission of the single tryptophan residues located at the dimer interface. The possible relevance of this structural transition in the Ca2+-independent membrane damaging activity is discussed.

References

Jan 1, 1977·Toxicon : Official Journal of the International Society on Toxinology·C C ChangJ Fohlman
Sep 1, 1979·European Journal of Biochemistry·C BonH Fraenkel-Conrat
Feb 1, 1991·Archives of Biochemistry and Biophysics·B FrancisI I Kaiser
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Jan 1, 1991·Methods in Enzymology·R L Heinrikson
Mar 1, 1990·Journal of Molecular Graphics·G Vriend
Dec 14, 1990·Science·D L ScottP B Sigler
Jan 1, 1989·Toxicon : Official Journal of the International Society on Toxinology·B Lomonte, J M Gutiérrez
Jan 1, 1988·Toxicon : Official Journal of the International Society on Toxinology·M I Homsi-BrandeburgoJ R Giglio
Nov 5, 1988·Journal of Molecular Biology·J JaninC Chothia
Jan 1, 1986·Toxicon : Official Journal of the International Society on Toxinology·D Mebs, Y Samejima
Oct 9, 1973·Biochemistry·E N Hudson, G Weber
Jan 1, 1984·Progress in Biophysics and Molecular Biology·E N Baker, R E Hubbard
Jun 14, 1982·Biochimica Et Biophysica Acta·M K JainG H De Haas
Apr 11, 1995·Biochemistry·J Z PedersenS Rufini
Jan 1, 1995·Methods in Enzymology·M K JainO G Berg
Nov 1, 1994·Toxicon : Official Journal of the International Society on Toxinology·B LomonteL A Hanson
Jul 5, 1994·Biochemical Pharmacology·A B MukherjeeN Pattabiraman
Jan 1, 1994·Advances in Protein Chemistry·D L Scott, P B Sigler
Sep 1, 1993·Biochemistry and Cell Biology = Biochimie Et Biologie Cellulaire·J M GerrardA McNicol
Aug 1, 1993·Toxicon : Official Journal of the International Society on Toxinology·R K Arni, J M Gutiérrez
Nov 3, 1993·Biochimica Et Biophysica Acta·I KudoK Inoue
Feb 1, 1993·Journal of Protein Chemistry·A C CintraJ R Giglio
Mar 1, 1993·Journal of Neurochemistry·J L BrusésG Pilar
Nov 1, 1995·Toxicon : Official Journal of the International Society on Toxinology·J M Gutiérrez, B Lomonte
Aug 1, 1996·Toxicon : Official Journal of the International Society on Toxinology·R K Arni, R J Ward
May 1, 1996·Revista Do Instituto De Medicina Tropical De São Paulo·C da C Lacaz

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Citations

Jun 21, 2001·Biochemical and Biophysical Research Communications·A H de OliveiraR J Ward
Mar 3, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·D P Marchi-SalvadorM R M Fontes
May 10, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Mário T MurakamiRaghuvir K Arni
Jun 7, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·D P Marchi-SalvadorM R M Fontes
Aug 2, 2006·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·L C CorrêaM R M Fontes
Dec 18, 2007·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·D P Marchi-SalvadorM R M Fontes
Aug 9, 2011·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Guilherme H M SalvadorMarcos R M Fontes
Apr 27, 2013·Chemical Reviews·Artur GoraJiri Damborsky
Oct 31, 2009·Toxicon : Official Journal of the International Society on Toxinology·M Gallacci, W L G Cavalcante
Jun 9, 2009·Toxicon : Official Journal of the International Society on Toxinology·Tatiana Lopes Ferreira, Richard J Ward
May 20, 2009·Toxicon : Official Journal of the International Society on Toxinology·Arthur H C de OliveiraRichard J Ward
Apr 30, 2009·Journal of Structural Biology·Juliana I dos SantosMarcos R M Fontes
Mar 24, 2009·Toxicon : Official Journal of the International Society on Toxinology·José María GutiérrezTeresa Escalante
Dec 28, 2007·Toxicon : Official Journal of the International Society on Toxinology·Elisangela Aparecida AragãoRichard J Ward
Jan 11, 2007·Biochemical and Biophysical Research Communications·Milton Roque BugsRichard John Ward
Dec 13, 2006·Toxicon : Official Journal of the International Society on Toxinology·Mário T MurakamiRaghuvir K Arni
Nov 19, 2003·Biochemical and Biophysical Research Communications·Angelo J MagroMarcos R M Fontes
Nov 13, 2004·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·A L CecchiniE C Arantes
Sep 2, 2006·Biophysical Chemistry·Raquel Kely Bortoleto-BugsR J Ward
Oct 4, 2005·International Journal of Biological Macromolecules·Milton Roque BugsMarinônio Lopes Cornélio
Jul 5, 2005·Toxicon : Official Journal of the International Society on Toxinology·Fábio K TicliSuely V Sampaio
Jun 23, 2005·Toxicon : Official Journal of the International Society on Toxinology·Yamileth AnguloBruno Lomonte
Jun 9, 2012·Toxicon : Official Journal of the International Society on Toxinology·Bruno Lomonte
Jul 3, 2013·Toxicon : Official Journal of the International Society on Toxinology·Guilherme H M SalvadorMarcos R M Fontes
Jul 12, 2012·Toxicon : Official Journal of the International Society on Toxinology·Bruno Lomonte, José Rangel
Dec 14, 2016·Medicinal Research Reviews·Sergio M MarquesJiri Damborsky
Jun 18, 2005·Journal of Molecular Biology·Mário T MurakamiRaghuvir K Arni
Feb 9, 2005·Chemico-biological Interactions·Walter L G CavalcanteMárcia Gallacci
Nov 16, 2017·Scientific Reports·Rafael J BorgesMarcos R M Fontes
Jan 20, 2011·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Marcelo L SantosMarcos H Toyama
Dec 15, 2004·The Journal of Biological Chemistry·Andre L B AmbrosioRichard C Garratt

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