Crystallographic evidence of a transglycosylation reaction: ternary complexes of a psychrophilic alpha-amylase

Biochemistry
N AghajariR Haser

Abstract

The psychrophilic Pseudoalteromonas haloplanctis alpha-amylase is shown to form ternary complexes with two alpha-amylase inhibitors present in the active site region, namely, a molecule of Tris and a trisaccharide inhibitor or heptasaccharide inhibitor, respectively. The crystal structures of these complexes have been determined by X-ray crystallography to 1.80 and 1.74 A resolution, respectively. In both cases, the prebound inhibitor Tris is expelled from the active site by the incoming oligosaccharide inhibitor substrate analogue, but stays linked to it, forming well-defined ternary complexes with the enzyme. These results illustrate competition in the crystalline state between two inhibitors, an oligosaccharide substrate analogue and a Tris molecule, bound at the same time in the active site region. Taken together, these structures show that the enzyme performs transglycosylation in the complex with the pseudotetrasaccharide acarbose (confirmed by a mutant structure), leading to a well-defined heptasaccharide, considered as a more potent inhibitor. Furthermore, the substrate-induced ordering of water molecules within a channel highlights a possible pathway used for hydrolysis of starch and related poly- and oligosaccharides.

References

Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·M Vihinen, P Mäntsälä
Jan 1, 1969·European Journal of Biochemistry·H Kersters-HildersonC K De Bruyne
May 27, 1994·Journal of Molecular Biology·A KadziolaR Haser
Sep 27, 1996·The Journal of Biological Chemistry·G FellerC Gerday
Oct 12, 1996·Annals of the New York Academy of Sciences·M Terashima, S Katoh
Jan 15, 1997·The Biochemical Journal·G J DaviesB Henrissat
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·A T Brünger

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Citations

Jan 16, 2003·Journal of Molecular Biology·Narayanan RamasubbuPrasunkumar J Mishra
Oct 20, 2005·Journal of Bioscience and Bioengineering·Anne HoyouxCharles Gerday
Apr 4, 2012·Journal of the American Chemical Society·Takahiro Kosugi, Shigehiko Hayashi
Dec 21, 2002·European Journal of Biochemistry·Hans LeemhuisLubbert Dijkhuizen
May 22, 2002·Protein Science : a Publication of the Protein Society·Nushin AghajariRichard Haser
Jun 17, 2008·Applied and Environmental Microbiology·Juanita Yazmin Damián-AlmazoGloria Saab-Rincón
Nov 18, 2003·Journal of Bacteriology·Masayuki KagawaHiroshi Mizuno
Oct 31, 2012·International Journal of Molecular Sciences·Caroline Struvay, Georges Feller
Jun 29, 2013·Cellular and Molecular Life Sciences : CMLS·Štefan JanečekE Ann MacGregor
Oct 9, 2012·Enzyme and Microbial Technology·Takashi TonozukaKoki Fujita
Mar 24, 2011·Biochemical and Biophysical Research Communications·Li-Chu TsaiLie-Fen Shyur
Feb 9, 2013·Bioscience, Biotechnology, and Biochemistry·Takayoshi TagamiAtsuo Kimura
Jun 9, 2004·European Journal of Biochemistry·Narayanan RamasubbuLili Kandra
Oct 28, 2008·Biotechnology and Bioengineering·Bassem KhemakhemSamir Bejar
May 8, 2016·Cellular and Molecular Life Sciences : CMLS·Štefan Janeček, Marek Gabriško
Aug 8, 2018·Journal of Chemical Ecology·Gladys Bichang'aPaul-André Calatayud
Jul 21, 2005·The Journal of Biological Chemistry·Xavier RobertNushin Aghajari
Dec 17, 2002·The Journal of Biological Chemistry·Anni LindenMatthias Wilmanns
Sep 4, 2015·The Journal of Biological Chemistry·Yuka OkazawaTakashi Tonozuka
May 28, 2020·Nature Communications·Jaka SočanJohan Åqvist
Sep 25, 2009·The Journal of Physical Chemistry. B·Marco PasiElena Papaleo
Jan 10, 2012·Journal of Chemical Theory and Computation·Takahiro Kosugi, Shigehiko Hayashi

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