Crystallographic studies of V44 mutants of Clostridium pasteurianum rubredoxin: effects of side-chain size on reduction potential

Proteins
Il Yeong ParkChulhee Kang

Abstract

Understanding the structural origins of differences in reduction potentials is crucial to understanding how various electron transfer proteins modulate their reduction potentials and how they evolve for diverse functional roles. Here, the high-resolution structures of several Clostridium pasteurianum rubredoxin (Cp Rd) variants with changes in the vicinity of the redox site are reported in order to increase this understanding. Our crystal structures of [V44L] (at 1.8 A resolution), [V44A] (1.6 A), [V44G] (2.0 A) and [V44A, G45P] (1.5 A) Rd (all in their oxidized states) show that there is a gradual decrease in the distance between Fe and the amide nitrogen of residue 44 upon reduction in the size of the side chain of residue 44; the decrease occurs from leucine to valine, alanine or glycine and is accompanied by a gradual increase in their reduction potentials. Mutation of Cp Rd at position 44 also changes the hydrogen-bond distance between the amide nitrogen of residue 44 and the sulfur of cysteine 42 in a size-dependent manner. Our results suggest that residue 44 is an important determinant of Rd reduction potential in a manner dictated by side-chain size. Along with the electric dipole moment of the 43-44 peptide bond and th...Continue Reading

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Apr 7, 2004·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Il Yeong ParkChulHee Kang

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Citations

Oct 4, 2005·Proceedings of the National Academy of Sciences of the United States of America·I-Jin LinJohn L Markley
Nov 23, 2013·Journal of Computer-aided Molecular Design·Jianzhong ChenGuohui Li
Aug 23, 2007·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Heiko BönischRudolf Ladenstein
May 1, 2008·Journal of Biological Inorganic Chemistry : JBIC : a Publication of the Society of Biological Inorganic Chemistry·Francesco BonomiEnzio Maria Ragg
May 23, 2006·Journal of Inorganic Biochemistry·David M LeMasterGriselda Hernández
Feb 9, 2012·Journal of the American Chemical Society·Peng ZhengHongbin Li
Jul 30, 2019·BioMed Research International·Ying LiXin Ni
Oct 13, 2009·Journal of Chemical Theory and Computation·Ana Patricia Gámiz-HernándezErnst-Walter Knapp
Mar 8, 2011·Journal of Chemical Theory and Computation·Ana Patricia Gamiz-HernandezE W Knapp

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