PMID: 11914072Mar 27, 2002Paper

Crystallographic study of the recombinant flavin-binding domain of Baker's yeast flavocytochrome b(2): comparison with the intact wild-type enzyme

Biochemistry
Louise M CunaneF Scott Mathews

Abstract

Flavocytochrome b(2) catalyzes the oxidation of L-lactate to pyruvate and the transfer of electrons to cytochrome c. The enzyme consists of a flavin-binding domain, which includes the active site for lacate oxidation, and a b(2)-cytochrome domain, required for efficient cytochrome c reduction. To better understand the structure and function of intra- and interprotein electron transfer, we have determined the crystal structure of the independently expressed flavin-binding domain of flavocytochrome b(2) to 2.50 A resolution and compared this with the structure of the intact enzyme, redetermined at 2.30 A resolution, both structures being from crystals cooled to 100 K. Whereas there is little overall difference between these structures, we do observe significant local changes near the interface region, some of which impact on amino acid side chains, such as Arg289, that have been shown previously to have an important role in catalysis. The disordered loop region found in flavocytochrome b(2) and its close homologues remain unresolved in frozen crystals of the flavin-binding domain, implying that the presence of the b(2)-cytochrome domain is not responsible for this positional disorder. The flavin-binding domain interacts poorly wi...Continue Reading

Citations

Apr 20, 2011·European Biophysics Journal : EBJ·Florence Lederer
Jun 24, 2003·Journal of Molecular Biology·Robert H H van den HeuvelAndrea Mattevi
Jan 8, 2010·Acta Crystallographica. Section F, Structural Biology and Crystallization Communications·Jean Marie BourhisYlva Lindqvist
Dec 14, 2012·PLoS Computational Biology·Alexander N Volkov, Nico A J van Nuland
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Oct 4, 2008·FEBS Letters·Salvatore PassarellaAnna Atlante
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May 26, 2016·Physical Chemistry Chemical Physics : PCCP·N GilletV Moliner
Jul 19, 2002·Yeast
Sep 13, 2018·Protein Science : a Publication of the Protein Society·Kelsey M Kean, P Andrew Karplus
Nov 6, 2014·Biomolecular Concepts·Elvira Romero, Giovanni Gadda

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