PMID: 9655943Jul 10, 1998Paper

Crystallography and mutagenesis of transketolase: mechanistic implications for enzymatic thiamin catalysis

Biochimica Et Biophysica Acta
G Schneider, Y Lindqvist

Abstract

The ThDP dependent enzyme transketolase is a convenient model system to study enzymatic thiamin catalysis. Crystallographic studies of the enzyme have identified the ThDP binding fold, the V-conformation of ThDP as the relevant conformation in enzymatic catalysis and details of enzyme-substrate interactions. Based on this structural information, the function of various active site residues in substrate binding and catalysis has been probed by site-directed mutagenesis.

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Citations

Jan 3, 2012·The Protein Journal·Olga N SolovjevaGerman A Kochetov
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·V YankiwskiN F Neff
Jul 30, 2010·The Journal of Biological Chemistry·Lars MitschkeKai Tittmann
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