Crystals of the Arp2/3 complex in two new space groups with structural information about actin-related protein 2 and potential WASP binding sites

Acta Crystallographica. Section F, Structural Biology Communications
Christopher T Jurgenson, Thomas D Pollard

Abstract

Co-crystals of the bovine Arp2/3 complex with the CA motif from N-WASP in two new space groups were analyzed by X-ray diffraction. The crystals in the orthorhombic space group P212121 contained one complex per asymmetric unit, with unit-cell parameters a = 105.48, b = 156.71, c = 177.84 Å, and diffracted to 3.9 Å resolution. The crystals in the tetragonal space group P41 contained two complexes per asymmetric unit, with unit-cell parameters a = b = 149.93, c = 265.91 Å, and diffracted to 5.0 Å resolution. The electron-density maps of both new crystal forms had densities for small segments of subdomains 1 and 2 of Arp2. Both maps had density at the binding site on Arp3 for the C-terminal EWE tripeptide from N-WASP and a binding site proposed for the C motif of N-WASP in the barbed-end groove of Arp2. The map from the tetragonal crystal form had density near the barbed end of Arp3 that may correspond to the C helix of N-WASP. The noise levels and the low resolution of the maps made the assignment of specific molecular structures for any of these CA peptides impossible.

References

May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·R D MullinsT D Pollard
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Mar 31, 1999·Proceedings of the National Academy of Sciences of the United States of America·L M MacheskyT D Pollard
Nov 27, 2001·Science·R C RobinsonT D Pollard
Oct 27, 2004·Proceedings of the National Academy of Sciences of the United States of America·Brad J NolenThomas D Pollard
Dec 2, 2004·Acta Crystallographica. Section D, Biological Crystallography·Paul Emsley, Kevin Cowtan
Nov 9, 2005·Proceedings of the National Academy of Sciences of the United States of America·David ChereauRoberto Dominguez
Dec 14, 2005·Current Opinion in Cell Biology·Theresia E B Stradal, Giorgio Scita
Sep 23, 2006·Nature Reviews. Molecular Cell Biology·Erin D Goley, Matthew D Welch
Jan 26, 2007·Proceedings of the National Academy of Sciences of the United States of America·Janna G KiselarMark R Chance
May 5, 2007·Annual Review of Biophysics and Biomolecular Structure·Thomas D Pollard
Mar 5, 2008·The Journal of Cell Biology·Isabelle RouillerDorit Hanein
May 9, 2008·Structure·Malgorzata BoczkowskaRoberto Dominguez
Jul 22, 2008·The Journal of Biological Chemistry·Brad J Nolen, Thomas D Pollard
Feb 4, 2010·Acta Crystallographica. Section D, Biological Crystallography·Paul D AdamsPeter H Zwart
Oct 21, 2010·Biophysical Journal·Paul Dalhaimer, Thomas D Pollard
Feb 18, 2011·Nature Reviews. Cancer·Alexander NürnbergRobert Grosse
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Martyn D WinnKeith S Wilson
Apr 5, 2011·Acta Crystallographica. Section D, Biological Crystallography·Garib N MurshudovAlexei A Vagin
Jun 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shih-Chieh TiThomas D Pollard
Jun 17, 2011·Proceedings of the National Academy of Sciences of the United States of America·Shae B PadrickMichael K Rosen
Oct 7, 2011·Cell Adhesion & Migration·Changsong Yang, Tatyana Svitkina
Dec 6, 2012·Nature Reviews. Molecular Cell Biology·Jeremy D RottyJames E Bear
Jan 11, 2013·Cell Adhesion & Migration·Stephane R Gross
Jul 31, 2013·Nature Structural & Molecular Biology·Qing Luan, Brad J Nolen
Sep 17, 2013·Cell Host & Microbe·Matthew D Welch, Michael Way
Feb 13, 2014·Nature Communications·Malgorzata BoczkowskaRoberto Dominguez
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor

❮ Previous
Next ❯

Citations

Jun 22, 2016·Proceedings of the National Academy of Sciences of the United States of America·Max Rodnick-SmithBrad J Nolen
Feb 2, 2018·Proceedings of the National Academy of Sciences of the United States of America·Qing LuanBrad J Nolen
Aug 26, 2020·Nature Structural & Molecular Biology·Mohammed ShaabanBrad J Nolen
Aug 29, 2018·Proceedings of the National Academy of Sciences of the United States of America·Sofia Espinoza-SanchezThomas D Pollard

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.

Related Papers

Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Leandra WatanabeIgor Polikarpov
Acta Crystallographica. Section F, Structural Biology and Crystallization Communications
Markus G RudolphDagmar Klostermeier
Proceedings of the National Academy of Sciences of the United States of America
Shih-Chieh TiThomas D Pollard
© 2021 Meta ULC. All rights reserved