CS-PEG decorated PLGA nano-prototype for delivery of bioactive compounds: A novel approach for induction of apoptosis in HepG2 cell line

Advances in Medical Sciences
Ahmed A Abd-Rabou, Hanaa H Ahmed

Abstract

Polymer-based nanoparticles are used as vectors for cancer drug delivery. The bioactive compounds (quercetin, ellagic acid and gallic acid) are well known to be not only antioxidants but also chemopreventive candidates against various types of cancers. To circumvent the low bioavailability and the short half-life time obstacles, we hypothesized a novel PLGA nano-platform functionalized with CS and PEG to encapsulate these phytochemicals. This encapsulation will protect the compounds from the phagocytic uptake and deliver PLGA-CS-PEG nano-prototype with high biodegradability and biosafety. Three consequent types of PLGA-based nanocomposites were prepared and characterized. Furthermore, we investigated the newly synthesized nano-formulations against human hepatocellular carcinoma (HepG2) and colorectal cancer (HCT 116) cell lines using cell growth inhibition assays, followed by apoptosis and necrosis assays using flow cytometry to detect the underlying mechanism of HepG2 cell death. Through Malvern Zeta Sizer, we recorded that the average diameters of the nano-prototypes ranged from 150 to 300nm. The cytotoxic activity of quercetin, ellagic acid, and gallic acid-encapsulated PLGA, PLGA-CS, and PLGA-CS-PEG nano-prototypes it has b...Continue Reading

Citations

Oct 27, 2017·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wenrui WangChangjie Chen
Sep 7, 2018·Journal of Cellular Biochemistry·Ammad Ahmad FarooqiBaojun Xu
Apr 25, 2019·Medicina·Firoozeh NiazvandAli Khodadadi
May 9, 2019·Biological Trace Element Research·Ahmed A Abd-RabouAziza B Shalby
Apr 14, 2020·International Journal of Nanomedicine·Deena S MousaShaker A Mousa
Jul 28, 2019·Applied Biochemistry and Biotechnology·Ahmed A Abd-RabouShaker A Mousa
Nov 15, 2019·Current Topics in Medicinal Chemistry·Sachin KumarHimangini Bansal
Jul 17, 2021·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mohamed Fawzi KabilIbrahim M El-Sherbiny

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