Csk-binding protein (Cbp) negatively regulates epidermal growth factor-induced cell transformation by controlling Src activation

Oncogene
L Q JiangZ Chen

Abstract

Epidermal growth factor receptor (EGFR) and Src tyrosine kinase cooperate in regulating EGFR-mediated cell signaling and promoting cell transformation and tumorigenesis in pathological conditions. Activation of Src is tightly regulated by the C-terminal Src kinase (Csk). The Csk-binding protein (Cbp) is a ubiquitously expressed transmembrane protein. Its functions include suppression of T-cell receptor activation through recruiting Csk and inhibiting Src family kinase (SFK). However, a potential role of Cbp in EGF-induced cell activities has not been investigated. Here, we report that EGF-stimulation-induced Cbp tyrosine phosphorylation followed by Cbp-Csk association, in a SFK-dependent manner. Expression of wild-type (wt) Cbp remarkably suppressed EGF-induced activation of Src, ERK1/2, and Akt-1 enzymes, and NIH3T3 cell transformation, as well as colony formation of a breast cancer cell line (MDA-MB-468) in soft agar. In contrast, expression of CbpY317F or knockdown endogenous Cbp in NIH3T3 cells by RNA interference significantly enhanced EGF-induced activation of these enzymes and cell transformation. In addition, overexpression of multiple receptor tyrosine kinases (RTKs)-induced Cbp tyrosine phosphorylation. These results ...Continue Reading

References

Oct 24, 1995·Proceedings of the National Academy of Sciences of the United States of America·E J SmartR G Anderson
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M C MaaS J Parsons
Jul 1, 1995·Breast Cancer Research and Treatment·W G Cance, E T Liu
Oct 9, 1996·International Journal of Cancer. Journal International Du Cancer·L TremblayS Chevalier
Jun 5, 1997·Nature·K Simons, E Ikonen
Aug 1, 1997·Biochemical Society Transactions·T NishinoH Nakazawa
Jun 8, 1999·The Journal of Biological Chemistry·M A OlayioyeN E Hynes
Jul 15, 1999·The Journal of Small Animal Practice·I K RamseyM E Herrtage
Dec 2, 1999·Experimental Cell Research·K A DeMaliA Kazlauskas
Mar 17, 2001·Nature Reviews. Molecular Cell Biology·Y Yarden, M X Sliwkowski
Aug 11, 2001·Biochemical Society Transactions·B RazaniM P Lisanti
Dec 5, 2002·The Journal of Biological Chemistry·Mei KongBarry I Posner
Feb 26, 2003·The Journal of Biological Chemistry·Tianyun Jiang, Yun Qiu
Mar 4, 2003·Molecular and Cellular Biology·Dominique DavidsonAndré Veillette
Jul 23, 2003·Molecular Psychiatry·J Z YangD Zhang
Oct 7, 2003·Oncogene·C BellanL Leoncini
Nov 14, 2003·The Journal of Biological Chemistry·Hidetada MatsuokaMasato Okada
Dec 3, 2003·Proceedings of the National Academy of Sciences of the United States of America·Takaki ShimaMasato Okada
Dec 10, 2003·Oncogene·Thomas F FrankeChizuru Sugimoto

❮ Previous
Next ❯

Citations

May 29, 2008·Cancer Metastasis Reviews·Jacky G GoetzIvan R Nabi
Feb 13, 2007·Current Opinion in Nephrology and Hypertension·Glaucia E CalleraRhian M Touyz
Jul 16, 2013·Oncogene·S KalakondaD V Kalvakolanu
Nov 12, 2013·Oncogene·M Hrdinka, V Horejsi
Aug 13, 2008·The Journal of Cell Biology·Laurence VeraciniChristine Benistant
Dec 24, 2013·Clinical Colorectal Cancer·Jiezhong ChenM Wasif Saif
Oct 11, 2015·Free Radical Biology & Medicine·Hongqiao Zhang, Henry Jay Forman
Jul 22, 2011·Journal of Signal Transduction·Banibrata Sen, Faye M Johnson
Jun 10, 2008·Cancer Cell·Marilyn D Resh
May 24, 2008·Molecular Cell·Chitose OneyamaMasato Okada
Sep 24, 2016·Cytokine & Growth Factor Reviews·Shreeram C Nallar, Dhan V Kalvakolanu
Mar 20, 2016·Oncotarget·Saurabh AgarwalJason M Shohet
Feb 23, 2007·Molecular Cancer Research : MCR·Michalis V KaramouzisAthanasios G Papavassiliou

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.