CSlo encodes calcium-activated potassium channels in the chick's cochlea

Proceedings. Biological Sciences
G J JiangPaul A Fuchs

Abstract

Large conductance, calcium-activated (BK) potassium channels play a central role in the excitability of cochlear hair cells. In mammalian brains, one class of these channels, termed Slo, is encoded by homologues of the Drosophila 'slowpoke' gene. By homology screening with mouse Sla cDNA, we have isolated a full-length clone (cSlo1) from a chick's cochlear cDNA library, rSlol had greater than 90% identity with mouse Slo at the amino acid level, and was even better matched to a human brain Slo at the amino and carboxy termini. cSlol had none of the additional exons found in splice variants from mammalian brain. The reverse transcriptase polymerase chain reaction (RT-PCR) was used to show expression of cSlal in the microdissected hair cell epithelium basilar papilla. Transient transfection of HIEK 293 cells demonstrated that cSlol encoded a potassium channel whose conductance averaged 224 pS at +60 mV in symmetrical 140 mM K. Macroscopic currents through cSlol channels were blocked by scorpion toxin or tetraethyl ammonium, and were voltage and calcium dependent. cSlol is likely to encode BK-type calcium-activated potassium channels in cochlear hair cells.

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Citations

May 4, 2005·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·T M MatthewsP A Fuchs
Apr 3, 1998·Biochimica Et Biophysica Acta·B DrozdowskiM I Lomax
Mar 30, 1999·Brain Research. Molecular Brain Research·M S RajeevanB H Sokolowski
Jul 22, 1999·Annals of the New York Academy of Sciences·E M JonesR Fettiplace
Apr 1, 1999·Annual Review of Physiology·R Fettiplace, P A Fuchs
Feb 1, 2011·Hearing Research·Zoe F Mann, Matthew W Kelley
Jul 24, 2004·The Journal of Physiology·Haresha SamaranayakeDhasakumar S Navaratnam
Nov 26, 1998·The Journal of Biological Chemistry·L DíazR Latorre

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