CspC regulates rpoS transcript levels and complements hfq deletions

Research in Microbiology
Ifat Cohen-OrEliora Z Ron

Abstract

The general stress response in Escherichia coli is activated by several stress agents, including entering the stationary growth phase. This response constitutes a complex regulatory network in which a large number of genes are induced and others are repressed. The stress response is regulated by the alternative sigma factor σ(S) encoded by the rpoS gene. The rpoS transcripts are substrates of the RNA binding protein, Hfq, which is essential for its translation. The rpoS mRNA is also a substrate of the cold shock protein C (CspC) which stabilizes the transcripts. Here we demonstrate, using pull-down assays, that CspC interacts with Hfq via mRNA molecules. We also show that CspC acts on the 5' UTR of the rpoS transcript, but its activity on rpoS is independent of Hfq. Moreover, we show that CspC suppresses the phenotypes of an hfq deletion. These results elucidate a new aspect in the post-transcriptional regulation of the stress response and will further our understanding of this complex network.

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Citations

Jun 7, 2011·Annual Review of Microbiology·Aurelia BattestiSusan Gottesman
Jan 26, 2016·Molecular Plant-microbe Interactions : MPMI·Lindsey Price Burbank, Drake Stenger
Mar 19, 2015·Frontiers in Microbiology·Elke Van AsscheHans P Steenackers
Aug 9, 2016·Frontiers in Microbiology·Riikka Keto-TimonenHannu Korkeala
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Jun 14, 2013·Environmental Microbiology Reports·Yotam ShenharEliora Z Ron
Jun 15, 2017·Proceedings of the National Academy of Sciences of the United States of America·Charlotte MichauxJörg Vogel
Dec 1, 2011·EcoSal Plus·Regine Hengge
Jul 15, 2021·Journal of Applied Microbiology·Evieann Cardoza, Harinder Singh

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