CsPI from the perianthless early-diverging Chloranthus spicatus show function on petal development in Arabidopsis thaliana

Botanical Studies
Kunmei SuZhiduan Chen

Abstract

In the floral ABC model, B-class genes comprised of DEFICIENS (DEF)/APETALA3 (AP3) and GLOBOSA (GLO)/PISTILLATA (PI) had been proposed to involve in second and third whorl floral organ development. However, less is known about the function of B-class genes from early-diverging angiosperms. Chloranthaceae is one of the early-diverging angiosperm families. In this study, we characterized the role of the PI-like gene CsPI cloned from Chloranthus spicatus which have the simplest perianthless bisexual flowers. The expression profile analysis reveals high levels of CsPI mRNA in stamens in Chloranthus spicatus, with weak distribution in leaves and other floral organs. Nevertheless, CsPI rescued both stamen and petal development in Arabidopsis thaliana pi-1 mutants and caused partially conversion of sepals into petaloid organs in wild-type Arabidopsis thaliana plants. Yeast two-hybrid analysis showed that CsPI can form not only homodimers but also heterodimers with proteins encoded by Arabidopsis thaliana and Chloranthus spicatus AP3-like genes. These results suggested that CsPI has an ancestral function on stamen development and that CsPI has capability to specify petal development in Arabidopsis thaliana. The finding indicates that t...Continue Reading

References

Jul 1, 1994·Genes & Development·K Goto, E M Meyerowitz
Mar 9, 1999·The Plant Journal : for Cell and Molecular Biology·S J Clough, A F Bent
Feb 13, 2001·Current Opinion in Plant Biology·G Theissen
Feb 24, 2001·Nature·G Theissen, H Saedler
Apr 19, 2002·Molecular Biology and Evolution·Kai-Uwe WinterGünter Theissen
May 16, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael J ZanisMichael J Donoghue
Oct 31, 2002·Plant & Cell Physiology·Hsing-Fun Hsu, Chang-Hsien Yang
May 15, 2003·Proceedings of the National Academy of Sciences of the United States of America·Rebecca S Lamb, Vivian F Irish
Aug 29, 2003·The Plant Journal : for Cell and Molecular Biology·Yingzhen YangThomas Jack
Nov 15, 2003·Molecular Phylogenetics and Evolution·Annette Becker, Günter Theissen
Dec 17, 2003·The Journal of Biological Chemistry·Tsai-Yu TzengChang-Hsien Yang
Nov 13, 2004·Development·Clinton J WhippleRobert J Schmidt
Aug 24, 2005·The Plant Journal : for Cell and Molecular Biology·Sangtae KimDouglas E Soltis
Nov 7, 2006·Development Genes and Evolution·Hongyan ShanZheng Meng
Mar 7, 2007·Journal of Experimental Botany·Hélène AdamJames W Tregear
Dec 1, 2007·Proceedings of the National Academy of Sciences of the United States of America·Michael J MooreDouglas E Soltis
Jan 13, 2012·Proceedings of the National Academy of Sciences of the United States of America·Cezary SmaczniakKerstin Kaufmann
Feb 9, 2012·International Journal of Molecular Sciences·Ling-Ling LvGuang-Ming Sun
Mar 13, 2013·Proceedings of the National Academy of Sciences of the United States of America·Rui ZhangHongzhi Kong

❮ Previous
Next ❯

Citations

Nov 19, 2019·Natural Product Research·Luis Miguel Guamán-OrtizAlírica I Suárez

❮ Previous
Next ❯

Methods Mentioned

BETA
PCR
two-hybrid
transgenic

Software Mentioned

Beacon Designer
CsPI

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.