CsrA regulation via binding to the base-pairing small RNA Spot 42.

Molecular Microbiology
Ying-Jung LaiTony Romeo

Abstract

The carbon storage regulator system and base-pairing small RNAs (sRNAs) represent two predominant modes of bacterial post-transcriptional regulation, which globally influence gene expression. Binding of CsrA protein to the 5' UTR or initial mRNA coding sequences can affect translation, RNA stability, and/or transcript elongation. Base-pairing sRNAs also regulate these processes, often requiring assistance from the RNA chaperone Hfq. Transcriptomics studies in Escherichia coli have identified many new CsrA targets, including Spot 42 and other base-pairing sRNAs. Spot 42 synthesis is repressed by cAMP-CRP, induced by the presence of glucose, and Spot 42 post-transcriptionally represses operons that facilitate metabolism of nonpreferred carbon sources. CsrA activity is also increased by glucose via effects on CsrA sRNA antagonists, CsrB/C. Here, we elucidate a mechanism wherein CsrA binds to and protects Spot 42 sRNA from RNase E-mediated cleavage. This protection leads to enhanced repression of srlA by Spot 42, a gene required for sorbitol uptake. A second, independent mechanism by which CsrA represses srlA is by binding to and inhibiting translation of srlM mRNA, encoding a transcriptional activator of srlA. Our findings demonst...Continue Reading

References

Jul 5, 1979·Journal of Molecular Biology·B G Sahagan, J E Dahlberg
Jan 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·P Babitzke, S R Kushner
Jul 1, 1987·Journal of Bacteriology·M Yamada, M H Saier
Oct 5, 1988·Journal of Molecular Biology·M Yamada, M H Saier
Dec 1, 1982·Journal of Bacteriology·C M Joyce, N D Grindley
Dec 8, 1995·The Journal of Biological Chemistry·N A SabnisT Romeo
Jan 1, 1994·Journal of Bacteriology·M KajitaniA Ishihama
Jun 1, 2000·Proceedings of the National Academy of Sciences of the United States of America·K A Datsenko, B L Wanner
Jan 24, 2002·Molecular Cell·Thorleif MøllerPoul Valentin-Hansen
Jul 9, 2002·Genes & Development·Thorleif MøllerPoul Valentin-Hansen
Jun 26, 2003·Nucleic Acids Research·Michael Zuker
Sep 17, 2003·Genes & Development·Eric MasséSusan Gottesman
Nov 19, 2003·Molecular Microbiology·Aixia ZhangSusan Gottesman
Nov 25, 2004·Journal of Molecular Biology·Richard A Lease, Sarah A Woodson
Nov 3, 2005·Genes & Development·Atilio Deana, Joel G Belasco
Dec 20, 2005·Molecular Microbiology·Maude Guillier, Susan Gottesman
Dec 14, 2006·Molecular Microbiology·Alexandra SittkaJörg Vogel
Mar 27, 2007·Current Opinion in Microbiology·Paul Babitzke, Tony Romeo
Mar 27, 2007·Current Opinion in Microbiology·Hiroji Aiba
Feb 26, 2009·Cell·Lauren S Waters, Gisela Storz
Jul 22, 2009·Journal of Molecular Biology·Jeffrey MercanteTony Romeo
Aug 8, 2009·Molecular Microbiology·Jörg Vogel
Aug 18, 2009·Molecular Microbiology·Martin OvergaardPoul Valentin-Hansen
Nov 3, 2009·Current Opinion in Microbiology·Kathrin S Fröhlich, Jörg Vogel
Feb 2, 2010·Journal of Bacteriology·Ricardo Gonzalez ChavezDimitris Georgellis
Mar 12, 2010·RNA Biology·Marie-Pier CaronEric Massé
Oct 29, 2010·Cold Spring Harbor Perspectives in Biology·Susan Gottesman, Gisela Storz
Dec 15, 2010·Nucleic Acids Research·Mark B SteadSidney R Kushner
Dec 30, 2010·Proceedings of the National Academy of Sciences of the United States of America·Razika Hussein, Han N Lim
Apr 15, 2011·Molecular Microbiology·Adrianne N EdwardsTony Romeo
Jul 16, 2011·Nature Reviews. Microbiology·Jörg Vogel, Ben F Luisi
Sep 20, 2011·Molecular Cell·Gisela StorzKaren M Wassarman
Mar 6, 2012·The EMBO Journal·Chase L BeiselGisela Storz

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Jul 11, 2021·Molecular Microbiology·Thomas Søndergaard Stenum, Erik Holmqvist

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