CT-1 induces angiogenesis by regulating the ADMA/DDAH Pathway

Biomedical Papers of the Medical Faculty of the University Palacký, Olomouc, Czechoslovakia
Zhen Zhong ZhengZhi Bing Guo

Abstract

Cardiotrophin-1 (CT-1), a member of the IL-6 superfamily, is elevated in the serum of patients with ischemic and valvular heart disease. In this study, we hypothesized that CT-1 induces endothelial cell angiogenesis and that the ADMA/DDAH pathway plays an important role in the process. pEGFP-N1-CTF1-GFP and pEGFP-N1 were constructed and used to transiently transfect to HUVECs, mediated by LipofectamineTM 2000. After transfection, the expression of CT-1 was examined by qRT-PCR and western blotting. Endothelial cell proliferation assay was evaluated using the 3-[4,5-dimethylthiazol-2-yl]-2,5 diphenyltetrazolium bromide (MTT) method. Migration assay was performed using transwell, tube formation test was examined on Matrigel, eNOSmRNA expression was assayed by qRT-PCR, DDAH I, DDAHII and VEGF expression were detected by western blotting, the level of ADMA and the activity of DDAH were measured by High Performance Liquid Chromatography, NOS activity and the concentration of NO were assayed by L-[3H] citrulline production from L-[3H]arginine. Overexpression of CT-1, increased endothelial cell proliferation, migration and formation of blood vessels, upregulated the expression of eNOSmRNA, DDAHI, DDAHII and VEGF, elevated the activity ...Continue Reading

References

Dec 17, 2003·Molecular and Cellular Biochemistry·Darren H FreedIan M C Dixon
Feb 22, 2005·Cardiovascular Research·Darren H FreedIan M C Dixon
Oct 4, 2006·Journal of Molecular and Cellular Cardiology·Natalia LópezMaría Antonia Fortuño
Oct 19, 2006·The International Journal of Biochemistry & Cell Biology·Zhen-Zhong Zheng, Zheng-Xiang Liu
Dec 23, 2006·Acta Pharmacologica Sinica·Zhen-Zhong Zheng, Zheng-Xiang Liu
Mar 4, 2008·Liver International : Official Journal of the International Association for the Study of the Liver·Zhen Fan YangSheung Tat Fan
Sep 26, 2009·Arteriosclerosis, Thrombosis, and Vascular Biology·Lorna R FiedlerBeata Wojciak-Stothard

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Citations

Sep 27, 2018·International Angiology : a Journal of the International Union of Angiology·Anna M WieczórDanuta Rość

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