PMID: 60276Mar 12, 1976Paper

CT banding of human chromosomes: description of the banding technique and some of its modifications

Human Genetics
J M Scheres

Abstract

A technique is described for staining centromeric areas and reverse, mainly telomeric bands in human chromosomes. With this "CT" technique karyotyping of C-banded metaphases is possible without previous or subsequent use of other banding methods. The method consists of an alkaline pretreatment at 60 degrees C with Ba(OH)2, followed by salt incubation in 2 X SSC at 60 degrees C and staining with the cationic dye "Stains-all". In a series of experiments the influence of the variables in the procedure was studied, with the following main results: 1) Ba(OH)2 treatment alone and subsequent staining produces a distinct reverse banding pattern in which the secondary constriction of chromosome 9 is positive. 2) The 2 X SSC incubation in the CT procedure causes the Ba(OH)2 induced reverse bands to become weaker; the centromeric regions, however, become very prominent. 3) If the temperature of the 2 X SSC treatment is raised to 85 degrees C, the CT technique results in a specific staining of the short arm regions of some probably variant acrocentric chromosomes. The interphase nuclei of individuals possessing such acrocentrics usually show very distinct chromocentres after this treatment; in the polymorphs these chromocentres are often s...Continue Reading

References

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Citations

Jan 1, 1982·Human Genetics·M JansenJ L Van den Brande
May 19, 1976·Human Genetics·J M Scheres, G F Merkx
Jun 24, 1977·Histochemistry·J M Scheres
Dec 1, 1982·Cancer Genetics and Cytogenetics·P H FitzgeraldM E Beard
Jan 1, 1983·Cancer Genetics and Cytogenetics·P H FitzgeraldM E Beard
Mar 15, 1985·Cancer Genetics and Cytogenetics·I PetkovićJ Konja
Feb 1, 1988·Cancer Genetics and Cytogenetics·W N HittelmanP Agbor
Jul 1, 1991·Cancer Genetics and Cytogenetics·I PetkovićJ Konja
Sep 1, 1991·Cancer Genetics and Cytogenetics·I Petković, M Cepulić
Feb 1, 1979·British Journal of Haematology·D K Hossfeld, S Köhler
Jan 1, 1979·American Journal of Medical Genetics·T W HustinxJ M Scheres
Aug 1, 1987·Clinical Genetics·R D TaalmanG B Stoelinga

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