Oct 26, 2018

CTCF looping is established during gastrulation in medaka embryos

BioRxiv : the Preprint Server for Biology
Ryohei NakamuraHiroyuki Takeda

Abstract

Genome architecture plays a critical role in gene regulation, but how the structures seen in mature cells emerge during embryonic development remains poorly understood. Here, we study early development in medaka (the Japanese killifish, Oryzias latipes) at 12 time points before, during, and after gastrulation which is the most dramatic event in early embryogenesis, and characterize transcription, protein binding, and genome architecture. We find that gastrulation is most associated with drastic changes in genome architecture, including the formation of the first loops between sites bound by the insulator protein CTCF and great increase in the size of contact domains. However, the position of CTCF is fixed throughout medaka embryogenesis. Interestingly, genome-wide transcription precedes the emergence of mature domains and CTCF-CTCF loops.

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Mentioned in this Paper

Embryo
Genome-Wide Association Study
Positioning Attribute
Size
Protein Binding
Genome
CTCF
Transcription, Genetic
CCCTC-binding factor
Cyprinodontidae

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