CTD-dependent and -independent mechanisms govern co-transcriptional capping of Pol II transcripts

Nature Communications
Melvin Noe GonzalezRonald C Conaway

Abstract

Co-transcriptional capping of RNA polymerase II (Pol II) transcripts by capping enzyme proceeds orders of magnitude more efficiently than capping of free RNA. Previous studies brought to light a role for the phosphorylated Pol II carboxyl-terminal domain (CTD) in activation of co-transcriptional capping; however, CTD phosphorylation alone could not account for the observed magnitude of activation. Here, we exploit a defined Pol II transcription system that supports both CTD phosphorylation and robust activation of capping to dissect the mechanism of co-transcriptional capping. Taken together, our findings identify a CTD-independent, but Pol II-mediated, mechanism that functions in parallel with CTD-dependent processes to ensure optimal capping, and they support a "tethering" model for the mechanism of activation.

References

Jan 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·S Shuman, J Hurwitz
Jan 1, 1996·Methods in Enzymology·R C ConawayJ W Conaway
Nov 1, 2002·Molecular Cell·Ya-Lin ChiuTariq M Rana
Nov 1, 2002·Molecular Cell·Shin Moteki, David Price
May 12, 2004·Proceedings of the National Academy of Sciences of the United States of America·Subhrangsu S MandalDanny Reinberg
Jun 20, 2008·Proceedings of the National Academy of Sciences of the United States of America·Ozan AygünYilun Liu
Aug 20, 2010·The Journal of Biological Chemistry·Man-Hee SuhJianhua Fu
Jun 21, 2011·Molecular Cell·Beate Schwer, Stewart Shuman
Jul 1, 2010·Wiley Interdisciplinary Reviews. RNA·Agnidipta Ghosh, Christopher D Lima
Oct 1, 2011·Wiley Interdisciplinary Reviews. RNA·Ivan TopisirovicAaron J Shatkin
Jul 24, 2012·Nature Structural & Molecular Biology·Matthew W KellingerDong Wang
Feb 26, 2013·Methods in Molecular Biology·Chieri Tomomori-SatoJoan W Conaway
Jul 22, 2014·Nature·Nicholas KwiatkowskiNathanael S Gray
May 12, 2015·Molecular Cell·Fuensanta W Martinez-RucoboPatrick Cramer
Aug 11, 2015·Molecular Cell·Kyle A NilsonDavid H Price
Jun 15, 2016·Annual Review of Biochemistry·Emmanuel Compe, Jean-Marc Egly
Jun 19, 2016·Nucleic Acids Research·Anand RamanathanSiu-Hong Chan
Aug 3, 2017·Cell Reports·Christopher C EbmeierDylan J Taatjes

❮ Previous
Next ❯

Citations

Jan 7, 2020·Bioscience Reports·Zaur M KachaevYulii V Shidlovskii
Oct 9, 2019·Proceedings of the National Academy of Sciences of the United States of America·Rina FujiwaraKenji Murakami
Jun 24, 2020·Nature Chemical Biology·Pabitra K Parua, Robert P Fisher
Aug 22, 2020·Annual Review of Cell and Developmental Biology·Sara Osman, Patrick Cramer
Apr 27, 2021·Nature·Shintaro AibaraPatrick Cramer
Mar 27, 2021·Annual Review of Biochemistry·Tim KrischunsStephen Cusack
Sep 2, 2021·Seminars in Immunopathology·Matteo Maurizio GuerriniYasuhiro Murakawa

❮ Previous
Next ❯

Methods Mentioned

BETA
electrophoresis
GTase
PCR

Software Mentioned

Graphpad Prism

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.