Ctf1, a transcriptional activator of cutinase and lipase genes in Fusarium oxysporum is dispensable for virulence.

Molecular Plant Pathology
Ana Lilia Martínez RochaM I Roncero

Abstract

Cutinolytic enzymes are secreted by fungal pathogens attacking the aerial parts of the plant, to facilitate penetration of the outermost cuticular barrier of the host. The role of cutinases in soil-borne root pathogens has not been studied thus far. Here we report the characterization of the zinc finger transcription factor Ctf1 from the vascular wilt fungus Fusarium oxysporum, a functional orthologue of CTF1alpha that controls expression of cutinase genes and virulence in the pea stem pathogen Fusarium solani f. sp. pisi. Mutants carrying a Deltactf1 loss-of-function allele grown on inducing substrates failed to activate extracellular cutinolytic activity and expression of the cut1 and lip1 genes, encoding a putative cutinase and lipase, respectively, whereas strains harbouring a ctf1(C) allele in which the ctf1 coding region was fused to the strong constitutive Aspergillus nidulans gpdA promoter showed increased induction of cutinase activity and gene expression. These results suggest that F. oxysporum Ctf1 mediates expression of genes involved in fatty acid hydrolysis. However, expression of lip1 during root infection was not dependent on Ctf1, and virulence of the ctf1 mutants on tomato plants and fruits was indistinguishab...Continue Reading

References

Jun 1, 1992·The Plant Cell·D J Stahl, W Schäfer
Jan 1, 1992·Methods in Enzymology·P J Punt, C A van den Hondel
Oct 5, 1990·Journal of Molecular Biology·S F AltschulD J Lipman
Jan 1, 1981·Methods in Enzymology·P E KolattukudyJ D Larson
May 9, 1995·Proceedings of the National Academy of Sciences of the United States of America·P E KolattukudyM A Flaishman
Jun 1, 1997·Fungal Genetics and Biology : FG & B·R B Todd, A Andrianopoulos
Oct 12, 1999·Molecular Plant-microbe Interactions : MPMI·T Roldán-ArjonaM Ruiz-Rubio
Dec 2, 2000·Biochimie·M R Egmond, J de Vlieg
Apr 25, 2001·Applied and Environmental Microbiology·F I García-MaceiraM I Roncero
Apr 4, 2002·Fungal Genetics and Biology : FG & B·E Gómez-GómezC Hera
Oct 19, 2002·Journal of Biotechnology·P E CruzM J T Carrondo
May 7, 2004·Applied and Environmental Microbiology·Joseph E Flaherty, Charles P Woloshuk
Apr 22, 2005·The Plant Journal : for Cell and Molecular Biology·Christian A VoigtSiegfried Salomon
Jul 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·C L SolidayP E Kolattukudy
Aug 29, 2007·Molecular Plant-microbe Interactions : MPMI·Fernando Calero-NietoConcepcion Hera
Sep 1, 2003·Molecular Plant Pathology·Antonio Di PietroM Isabel G Roncero

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Citations

Jul 20, 2011·Molecular & Cellular Proteomics : MCP·Rodrigo Duarte GonçalvesMaria Célia Bertolini
May 31, 2013·Molecular Plant-microbe Interactions : MPMI·Gustavo Bravo-RuizM Isabel G Roncero
Apr 30, 2009·Molecular Plant Pathology·Caroline B Michielse, Martijn Rep
Sep 24, 2013·Biotechnology Advances·Sheng ChenJing Wu
Aug 19, 2016·G3 : Genes - Genomes - Genetics·Xingyu LuoNancy P Keller
Mar 14, 2019·Applied Microbiology and Biotechnology·Eva Bermúdez-GarcíaAmelia Farrés
Jan 13, 2021·International Journal of Biological Macromolecules·Shuaifeng GuXianghui Zhao
May 11, 2021·Molecular Plant Pathology·Qussai ZuriegatYingzi Yun

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