CTGF upregulation correlates with MMP-9 level in airway remodeling in a murine model of asthma

Archives of Medical Science : AMS
Sheng-Chieh LinChung-Ming Chen

Abstract

Connective tissue growth factor (CTGF) mediates hypertrophy, proliferation, and extracellular matrix synthesis. Matrix metalloproteinase (MMP) plays a role in airway extracellular matrix remodeling. The correlation between CTGF and MMP in airway remodeling of asthma was unknown. This study investigated lung CTGF expression and its correlation with MMP and airway structural changes in a murine model of asthma. Female BALB/c mice were sensitized and challenged by intraperitoneal injections and intranasal phosphate-buffered saline (PBS) or ovalbumin (OVA). Airway responsiveness and serum OVA-specific IgE were measured. Airway structural changes were quantified by morphometric analysis. Differential cell counts and MMP-2, MMP-9, and tissue inhibitor of metalloproteinase (TIMP)-1 were evaluated in bronchoalveolar lavage fluid (BALF). Lung CTGF was determined by Western blot. Serum OVA-specific IgE level and airway responsiveness in enhanced pause (Penh) is significantly higher in sensitized mice challenged with OVA compared to PBS-challenged mice. MMP-2, MMP-9, and TIMP-1 in BALF were significantly higher in OVA mice. Airway structural changes of animals' lungs with OVA challenge showed increased thickness of the smooth muscle layer...Continue Reading

Citations

Jun 21, 2020·Clinical and Experimental Immunology·J WieczfinskaR Pawliczak
Aug 3, 2017·Asia Pacific Allergy·Yang Ki KimChoon-Sik Park
Oct 27, 2018·BioMed Research International·Anelize Sartori Santos BortolozzoIolanda de Fátima Lopes Calvo Tibério
Jun 18, 2018·Biomaterials·Paul M GeorgeGary K Steinberg

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Methods Mentioned

BETA
biopsy
ELISA
bronchoalveolar
lavage

Software Mentioned

AIDA

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