Cth2 Protein Mediates Early Adaptation of Yeast Cells to Oxidative Stress Conditions

PloS One
Laia Castells-RocaEnrique Herrero

Abstract

Cth2 is an mRNA-binding protein that participates in remodeling yeast cell metabolism in iron starvation conditions by promoting decay of the targeted molecules, in order to avoid excess iron consumption. This study shows that in the absence of Cth2 immediate upregulation of expression of several of the iron regulon genes (involved in high affinity iron uptake and intracellular iron redistribution) upon oxidative stress by hydroperoxide is more intense than in wild type conditions where Cth2 is present. The oxidative stress provokes a temporary increase in the levels of Cth2 (itself a member of the iron regulon). In such conditions Cth2 molecules accumulate at P bodies-like structures when the constitutive mRNA decay machinery is compromised. In addition, a null Δcth2 mutant shows defects, in comparison to CTH2 wild type cells, in exit from α factor-induced arrest at the G1 stage of the cell cycle when hydroperoxide treatment is applied. The cell cycle defects are rescued in conditions that compromise uptake of external iron into the cytosol. The observations support a role of Cth2 in modulating expression of diverse iron regulon genes, excluding those specifically involved in the reductive branch of the high-affinity transport...Continue Reading

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Citations

Jun 20, 2019·G3 : Genes - Genomes - Genetics·Melinda HauserJeffrey M Becker
Sep 22, 2020·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·Xiang SunShizhong Zhang
Dec 7, 2019·The Journal of Biological Chemistry·Sabrina S SchatzmanValeria C Culotta
Aug 22, 2018·Current Genetics·Lucía Ramos-AlonsoMaría Teresa Martínez-Pastor

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Datasets Mentioned

BETA
E-MTAB-4122

Methods Mentioned

BETA
environmental stresses
PCR
flow cytometry
Fluorescence microscopy
FACS

Software Mentioned

Regulatory Sequence Analysis Tools ( RSAT )
cluster
ArrayExpress
Genepix Pro
CARMAweb

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