CTL epitope distribution patterns in the Gag and Nef proteins of HIV-1 from subtype A infected subjects in Kenya: use of multiple peptide sets increases the detectable breadth of the CTL response.

BMC Immunology
Jeffrey R CurrierJosephine H Cox

Abstract

Subtype A is a major strain in the HIV-1 pandemic in eastern Europe, central Asia and in certain regions of east Africa, notably in rural Kenya. While considerable effort has been focused upon mapping and defining immunodominant CTL epitopes in HIV-1 subtype B and subtype C infections, few epitope mapping studies have focused upon subtype A. We have used the IFN-gamma ELIspot assay and overlapping peptide pools to show that the pattern of CTL recognition of the Gag and Nef proteins in subtype A infection is similar to that seen in subtypes B and C. The p17 and p24 proteins of Gag and the central conserved region of Nef were targeted by CTL from HIV-1-infected Kenyans. Several epitope/HLA associations commonly seen in subtype B and C infection were also observed in subtype A infections. Notably, an immunodominant HLA-C restricted epitope (Gag 296-304; YL9) was observed, with 8/9 HLA-CW0304 subjects responding to this epitope. Screening the cohort with peptide sets representing subtypes A, C and D (the three most prevalent HIV-1 subtypes in east Africa), revealed that peptide sets based upon an homologous subtype (either isolate or consensus) only marginally improved the capacity to detect CTL responses. While the different pepti...Continue Reading

References

Aug 13, 1998·Nature Medicine·F KernH D Volk
Apr 20, 2001·Nature·A J McMichael, S L Rowland-Jones
May 29, 2001·Nature Immunology·B D Walker, B T Korber
Jun 29, 2002·Science·Brian GaschenBette Korber
Apr 2, 2003·Journal of Immunological Methods·Rika DraenertBruce D Walker
Jun 28, 2003·Science·Richard D KlausnerJudith N Wasserheit
Jul 2, 2003·Nature Medicine·Andrew J McMichael, Tomas Hanke
Nov 5, 2003·Nature Medicine·Christian Brander, Bruce D Walker
Feb 27, 2004·Nature Immunology·Dennis R BurtonRichard T Wyatt
Apr 2, 2004·Expert Review of Vaccines·Vasso Apostolopoulos, Eliada Lazoura
Aug 3, 2004·Expert Review of Vaccines·Feng GaoBarton F Haynes
Dec 14, 2004·Nature·Photini KiepielaPhilip J R Goulder
Oct 18, 2005·AIDS Research and Human Retroviruses·Megan C SteainNitin K Saksena
Oct 27, 2005·Journal of Acquired Immune Deficiency Syndromes : JAIDS·Lyle R McKinnonFrancis A Plummer
Dec 29, 2005·AIDS Research and Human Retroviruses·Stephanie RainwaterJulie Overbaugh

❮ Previous
Next ❯

Citations

Oct 27, 2007·AIDS Research and Human Retroviruses·Adam C FinnefrockJon H Condra
Oct 16, 2012·AIDS Research and Human Retroviruses·Sajib ChakrabortyMunsi Sahiuzzaman
Sep 8, 2007·The Journal of Clinical Investigation·Aleksandra LeligdowiczSarah Rowland-Jones
Aug 21, 2009·Clinical and Vaccine Immunology : CVI·Xiao-Li HuangCharles R Rinaldo
Dec 14, 2007·Clinical and Vaccine Immunology : CVI·Jeffrey R CurrierJosephine H Cox
Nov 16, 2010·The Journal of Immunology : Official Journal of the American Association of Immunologists·Alon HerschhornAmnon Hizi
Mar 21, 2008·The Journal of Immunology : Official Journal of the American Association of Immunologists·Carina L PérezAnnika C Karlsson
May 21, 2014·Molecular Biology Reports·Nazli JafarpourMehdi Mahdavi

❮ Previous
Next ❯

Datasets Mentioned

BETA
AY945736

Methods Mentioned

BETA
flow cytometry
ELISA
PCR

Software Mentioned

KS
TreeStar
SEQBOOT
PHYLIP
Excel
DNADIST
FlowJo

Related Concepts

Related Feeds