Cu-complex counteracts the effect of paraquat on polymerized actin

Toxicology in Vitro : an International Journal Published in Association with BIBRA
M Urbancíková, P Korytár

Abstract

The copper complex tetraanhydroaminobenzaldehyde is a synthetic compound with structural (16 atoms in a macrocyclic ring, four donor nitrogens associated with Cu(2+)) and functional similarity to the enzyme superoxide dismutase. Its ability to catalyse superoxide anion to molecular oxygen and hydrogen peroxide makes this complex interesting since it may be used to avoid or at least reduce different kinds of injuries induced by oxidative stress. To confirm this hypothesis at the cell level we used human hepatoma cell line HepG2 and a widespread herbicide, paraquat, known to induce production of free oxygen radicals through its reduction/oxidation as the model system. One of target sites described for paraquat is actin cytoskeleton. This phenomenon was used to detect and to characterize the possible ability of the Cu-complex to influence the effect of paraquat on actin structures. We investigated the Cu-complex in three different conditions: (1) the simultaneous effect of paraquat and the Cu-complex; (2) pretreatment with the Cu-complex following paraquat exposure; (3) exposure to paraquat following the treatment with Cu-complex. Phalloidin, conjugated with fluorescein isothiocyanate, known to bind specifically to filamentous act...Continue Reading

References

Mar 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·R PollackK Weber
Jun 4, 1974·Biochemical and Biophysical Research Communications·J S BusJ E Gibson
Jun 1, 1995·Journal of Inorganic Biochemistry·Z Duracková, J Labuda

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Citations

Oct 25, 2002·Toxicology in Vitro : an International Journal Published in Association with BIBRA·I DovinováE Tibenská
Nov 6, 2003·Toxicology in Vitro : an International Journal Published in Association with BIBRA·A Gómez-Mendikute, M P Cajaraville

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