Cucumber mosaic virus is restricted from entering minor veins in transgenic tobacco exhibiting replicase-mediated resistance

Virology
W M WintermantelM Zaitlin

Abstract

Transgenic tobacco plants expressing an altered form of the 2a replicase gene from cucumber mosaic virus (CMV) strain Fny exhibited a suppression of viral replication and restricted viral movement when inoculated mechanically or by insect vectors. Resistant plants could be infected, however, through a graft-union with an infected nontransformed plant. The infectious entity moved quickly through intergrafts of resistant tissue, indicating that it could move without replicating in the vascular system. Viral replication continued to be suppressed in systemically infected transgenic portions of grafted plants, as demonstrated by the synthesis of lower levels of viral RNA than in systemically infected nontransformed portions of the same grafted plants. Cell-to-cell spread within this tissue also occurred much more slowly than in nontransformed tobacco. Young inoculated levels of transgenic-resistant plants exhibited limited cell-to-cell virus movement, revealed as chlorotic lesions, but no long-distance virus movement occurred. The results of in situ hybridization studies on these lesions indicated that CMV RNA does not traffic from bundle-sheath cells to vascular parenchyma or companion cells in chlorotic lesions on the inoculated ...Continue Reading

References

Sep 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·J M AndersonM Zaitlin
Jan 1, 1992·Advances in Virus Research·P PalukaitisR I Francki
Feb 1, 1995·Journal of Virology·M E Taliansky, F García-Arenal
Oct 29, 1996·Proceedings of the National Academy of Sciences of the United States of America·L NguyenM Zaitlin
Jan 1, 1997·Advances in Virus Research·P Palukaitis, M Zaitlin
Sep 1, 1949·The Annals of Applied Biology·S P CAPOOR
Jan 1, 1995·Annual Review of Phytopathology·G P Lomonossoff

❮ Previous
Next ❯

Citations

Jul 16, 2008·Molecular Plant-microbe Interactions : MPMI·Marco MorroniMark Tepfer
Jul 12, 2008·Molecular Plant-microbe Interactions : MPMI·Tefera MekuriaJeanmarie Verchot-Lubicz
Aug 17, 2002·Molecular Plant-microbe Interactions : MPMI·Ezequiel Balmori-MelianRichard L S Forster
Sep 19, 2002·Molecular Plant-microbe Interactions : MPMI·Bu-Jun ShiRobert H Symons
Sep 16, 2004·Journal of Virology·Hongye Li, Marilyn J Roossinck
Aug 5, 2005·Annual Review of Phytopathology·Byoung-Cheorl KangMolly M Jahn
Aug 17, 2005·Proceedings of the National Academy of Sciences of the United States of America·Shoko Ueki, Vitaly Citovsky
Feb 2, 1999·Molecular Plant-microbe Interactions : MPMI·L Simón-Buela, F García-Arenal
Sep 6, 2011·Plant Science : an International Journal of Experimental Plant Biology·Anssi L VuorinenJari P T Valkonen
Aug 19, 2008·Molecular Plant Pathology·Blanca Gosalvez-BernalM Amelia Sanchez-Pina
Feb 17, 2000·The Journal of General Virology·W M Wintermantel, M Zaitlin
Dec 15, 2015·Molecular Plant Pathology·Cèlia Guiu-AragonésAna Montserrat Martín-Hernández
Aug 19, 2008·Molecular Plant Pathology·Marcel PrinsMark Tepfer
Aug 1, 2000·Plant Disease·Rebecca C GrubeMolly K Jahn

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.