Cucurbiturils: molecular nanocapsules for time-resolved fluorescence-based assays

IEEE Transactions on Nanobioscience
Cesar MarquezWerner M Nau

Abstract

A new fluorescent host-guest system based on the inclusion of the fluorophore 2,3-diazabicyclo[2.2.2]oct-2-ene (DBO) into the cavity of the molecular container compound cucurbit[7]uril (CB7) has been designed which possesses an exceedingly long-lived emission (690 ns in aerated water). The large binding constant of (4 +/- 1) x 10(5) M(-1) along with the resistance of the CB7.DBO complex toward external fluorescence quenchers allow the use of CB7 as an enhancer in time-resolved fluorescence-based assays, e.g., to screen enzyme activity or inhibition by using DBO-labeled peptides as substrates. The response of CB7.DBO to different environmental conditions and possible quenchers are described.

References

Nov 28, 2001·The Journal of Organic Chemistry·A DayB Snushall
Feb 19, 2002·Chemical Reviews·József Szejtli
Sep 19, 2002·Journal of the American Chemical Society·Dieter KlapsteinWerner M Nau
Oct 10, 2002·Chemical Reviews·Rieko van HeerbeekJoost N H Reek
Dec 6, 2002·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Werner M Nau, Xiaojuan Wang
May 29, 2003·Angewandte Chemie·Fang Huang, Werner M Nau
Oct 11, 2003·Organic Letters·Cesar MarquezWerner M Nau
Jan 22, 2004·Journal of the American Chemical Society·Xiaojuan Wang, Werner M Nau

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Citations

Nov 19, 2013·International Journal of Pharmaceutics·Na'il SalehMohammed Meetani
Mar 7, 2012·Journal of Molecular Modeling·Jayshree K KhedkarShridhar P Gejji
Mar 24, 2011·Future Medicinal Chemistry·Nial J WheateOliver B Sutcliffe
Jul 22, 2014·Chemistry : a European Journal·Julián VázquezUwe Pischel
Feb 14, 2012·Advanced Drug Delivery Reviews·Indrajit Ghosh, Werner M Nau
Jul 30, 2005·Angewandte Chemie·Jason LagonaLyle Isaacs
May 12, 2005·Angewandte Chemie·Jyotirmayee Mohanty, Werner M Nau
Dec 16, 2006·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jyotirmayee MohantyWerner M Nau
Mar 11, 2006·Chembiochem : a European Journal of Chemical Biology·Andreas HennigWerner M Nau
May 15, 2012·Chemistry : a European Journal·M PessêgoL Garcia-Rio
Sep 15, 2010·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jyotirmayee MohantyHaridas Pal
Jun 24, 2010·Chemistry : a European Journal·Mantosh K SinhaEhud Keinan
Jan 13, 2011·Chemistry : a European Journal·Jinshui LiuXianfeng Zhang
Feb 27, 2013·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Martin F Czar, Rebecca A Jockusch
Aug 29, 2012·Chemphyschem : a European Journal of Chemical Physics and Physical Chemistry·Jesper R NilssonUwe Pischel
Nov 12, 2010·Angewandte Chemie·Apurba L KonerWerner M Nau
Feb 7, 2015·The Journal of Physical Chemistry. B·Meenakshi N ShindeJyotirmayee Mohanty
Apr 4, 2008·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Mhejabeen ShaikhHaridas Pal
Dec 19, 2013·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Zsombor MiskolczyCatherine Amiel
Apr 29, 2016·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Barbara HeldFrank H Quina
Jul 22, 2016·The Journal of Physical Chemistry. B·Na'il SalehPanče Naumov
Mar 6, 2012·The Journal of Physical Chemistry. B·Nilotpal BarooahAchikanath C Bhasikuttan
Jun 3, 2014·The Journal of Physical Chemistry. B·Nilotpal BarooahAchikanath C Bhasikuttan
Apr 26, 2011·Journal of the American Chemical Society·Garima GhaleWerner M Nau
Jul 18, 2012·Journal of the American Chemical Society·Brittany VinciguerraLyle Isaacs
Aug 7, 2012·Organic Letters·Anuradha SinghRonald L Halterman
Feb 10, 2012·Journal of the American Chemical Society·Marina FreitagElena Galoppini
Nov 30, 2016·Chemistry, an Asian Journal·Nilotpal BarooahJyotirmayee Mohanty
Mar 12, 2010·Chemical Communications : Chem Comm·Dezhi JiaoOren A Scherman
Dec 22, 2009·Photochemical & Photobiological Sciences : Official Journal of the European Photochemistry Association and the European Society for Photobiology·Roland MeyerWerner M Nau
Dec 3, 2016·Chemistry : a European Journal·Stephan SinnLuisa De Cola
May 4, 2018·Nanoscale·Anne UlfkjærMichael Pittelkow
Apr 20, 2010·Organic & Biomolecular Chemistry·Vanya D UzunovaAnthony I Day
May 13, 2010·Chemical Communications : Chem Comm·Uwe PischelWerner M Nau
Jun 29, 2010·Physical Chemistry Chemical Physics : PCCP·Sumeet MahajanOren A Scherman
Jun 30, 2011·Chemical Communications : Chem Comm·Achikanath C BhasikuttanJyotirmayee Mohanty

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