Cuf2 is a novel meiosis-specific regulatory factor of meiosis maturation.

PloS One
Raphaël IoannoniSimon Labbé

Abstract

Meiosis is the specialized form of the cell cycle by which diploid cells produce the haploid gametes required for sexual reproduction. Initiation and progression through meiosis requires that the expression of the meiotic genes is precisely controlled so as to provide the correct gene products at the correct times. During meiosis, four temporal gene clusters are either induced or repressed by a cascade of transcription factors. In this report a novel copper-fist-type regulator, Cuf2, is shown to be expressed exclusively during meiosis. The expression profile of the cuf2(+) mRNA revealed that it was induced during middle-phase meiosis. Both cuf2(+) mRNA and protein levels are unregulated by copper addition or starvation. The transcription of cuf2(+) required the presence of a functional mei4(+) gene encoding a key transcription factor that activates the expression of numerous middle meiotic genes. Microscopic analyses of cells expressing a functional Cuf2-GFP protein revealed that Cuf2 co-localized with both homologous chromosomes and sister chromatids during the meiotic divisions. Cells lacking Cuf2 showed an elevated and sustained expression of several of the middle meiotic genes that persisted even during late meiosis. Moreov...Continue Reading

References

Jul 27, 1992·FEBS Letters·B Halliwell, J M Gutteridge
Jan 1, 1991·Methods in Enzymology·S MorenoP Nurse
Oct 17, 1994·Molecular & General Genetics : MGG·J Jimenez, J Oballe
Oct 6, 1997·Progress in Nucleic Acid Research and Molecular Biology·D R Winge
Jan 27, 1998·The Journal of Biological Chemistry·Z ZhuD J Thiele
Dec 14, 1999·The Journal of Biological Chemistry·S LabbéD J Thiele
Jul 19, 2001·Proceedings of the National Academy of Sciences of the United States of America·L Davis, G R Smith
Feb 23, 2002·Nature·V WoodL Cerrutti
Apr 17, 2002·The Journal of Biological Chemistry·Benoit PelletierSimon Labbé
Aug 6, 2002·Nature Genetics·Juan MataJürg Bähler
Sep 24, 2002·The Journal of Biological Chemistry·Daniel R BellemareSimon Labbe
Jan 17, 2003·Molecular Biology of the Cell·Dongrong ChenJürg Bähler
Jun 26, 2004·Cytogenetic and Genome Research·H M CritchlowD K Griffin
Dec 2, 2004·Nature Reviews. Molecular Cell Biology·Adèle L Marston, Angelika Amon
Feb 10, 2006·Eukaryotic Cell·Jude Beaudoin, Simon Labbé
Oct 13, 2006·Proceedings of the National Academy of Sciences of the United States of America·Juan Mata, Jürg Bähler
Aug 4, 2007·Chromosome Research : an International Journal on the Molecular, Supramolecular and Evolutionary Aspects of Chromosome Biology·Yuriko Harigaya, Masayuki Yamamoto
Jun 14, 2008·Molecular Biology of the Cell·Taro NakamuraChikashi Shimoda
Jan 7, 2010·Nature Reviews. Genetics·Mary Ann Handel, John C Schimenti
Apr 7, 2010·The Journal of Clinical Investigation·Cathryn A Hogarth, Michael D Griswold
Apr 23, 2010·Molecular Biology of the Cell·Akira ShigehisaHiroshi Nojima
Jun 10, 2010·Molecular Systems Biology·Maria J AmorimJuan Mata
Aug 10, 2010·Nature Chemical Biology·Alison M KimTeresa K Woodruff
Aug 10, 2010·PloS One·Raphaël IoannoniSimon Labbé

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Citations

Jul 11, 2014·Metallomics : Integrated Biometal Science·E Yu IlyechovaL V Puchkova
Feb 12, 2013·Eukaryotic Cell·Jude BeaudoinSimon Labbé
Oct 17, 2015·PloS One·Yulia A ZatulovskaiaLudmila V Puchkova
Feb 27, 2014·The Journal of Biological Chemistry·Samuel PlanteSimon Labbé
Aug 1, 2015·The Journal of Biological Chemistry·Vincent NormantSimon Labbé

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