Cu(II) nonspecifically binding chromate reductase NfoR promotes Cr(VI) reduction.

Environmental Microbiology
Huawen HanXiangkai Li

Abstract

Cu(II)-enhanced microbial Cr(VI) reduction is common in the environment, yet its mechanism is unknown. The specific activity of chromate reductase, NfoR, from Staphylococcus aureus sp. LZ-01 was augmented 1.5-fold by Cu(II). Isothermal titration calorimetry and spectral data show that Cu(II) binds to NfoR nonspecifically. Further, Cu(II) stimulates the nitrobenzene reduction of NfoR, indicating that Cu(II) promotes electron transfer. The crystal structure of NfoR in complex with CuSO4 (1.46 Å) was determined. The overall structure of NfoR-Cu(II) complex is a dimer that covalently binds with FMN and Cu(II)-binding pocket is located at the interface of the NfoR dimer. Structural superposition revealed that NfoR resembles the structure of class II chromate reductase. Site-directed mutagenesis revealed that Leu46 and Phe123 were involved in NADH binding, whereas Trp70 and Ser45 were the key residues for nitrobenzene binding. Furthermore, His100 and Asp171 were preferential affinity sites for Cu(II) and that Cys163 is an active site for FMN binding. Attenuation reductase activity in C163S can be partially restored to 54% wild type by increasing Cu(II) concentration. Partial restoration indicates dual-channel electron transfer of Nfo...Continue Reading

References

Jan 1, 1997·Journal of Receptor and Signal Transduction Research·G Klebe, H J Böhm
Mar 1, 2000·Trends in Biochemical Sciences·M W Fraaije, A Mattevi
May 2, 2000·Applied and Environmental Microbiology·C H ParkA Matin
Sep 27, 2000·Biochimica Et Biophysica Acta·A D NielsenP Westh
Feb 28, 2002·Proceedings of the National Academy of Sciences of the United States of America·Sue A RobertsWilliam R Montfort
Mar 15, 2003·The Journal of Biological Chemistry·Francisco J EnguitaMaria Arménia Carrondo
Apr 8, 2003·Applied Microbiology and Biotechnology·F A O CamargoW T Frankenberger
Jul 16, 2003·Applied Microbiology and Biotechnology·B ChardinM Bruschi
Aug 7, 2003·Applied and Environmental Microbiology·Young Hak KwakHan Bok Kim
Nov 24, 2004·Current Opinion in Chemical Biology·Brian E Collins, James C Paulson
Apr 21, 2005·The Journal of Biological Chemistry·Claudio F GonzalezA Matin
Oct 19, 2005·Biochemistry·Alexander MorokuttiPeter Macheroux
Apr 5, 2007·Nature Protocols·Adrian Velazquez-Campoy, Ernesto Freire
Aug 4, 2007·FEBS Letters·Jun YeHiranmoy Bhattacharjee
Feb 12, 2008·Journal of Bacteriology·Diederik Johannes OppermanEsta van Heerden
Mar 22, 2008·FEMS Microbiology Reviews·María Dolores RoldánConrado Moreno-Vivián
Feb 9, 2010·Biochemical and Biophysical Research Communications·Diederik J OppermanEsta van Heerden
May 8, 2010·Organic & Biomolecular Chemistry·Yanto YantoAndreas S Bommarius
May 27, 2010·PloS One·Chris W DiehneltStephen Albert Johnston
Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Margaret E Johnson, Gerhard Hummer
Sep 23, 2011·Applied Microbiology and Biotechnology·Yiguo HongShunshan Duan
Nov 15, 2011·Current Biology : CB·Richard A Festa, Dennis J Thiele
Feb 7, 2012·Biochimica Et Biophysica Acta·Maria E Palm-EsplingPernilla Wittung-Stafshede
Aug 7, 2012·Proteins·Olexandr IsayevJerzy Leszczynski
Sep 18, 2012·Environmental Science and Pollution Research International·Sumit K SoniAlok Kalra
Aug 16, 2014·Preparative Biochemistry & Biotechnology·Anuradha Hora, Vidya Shetty K
Nov 27, 2015·Journal of Agricultural and Food Chemistry·Maarit KaronenRebecca Joanne Green
Dec 4, 2015·Proceedings of the National Academy of Sciences of the United States of America·Parisa HosseinzadehYi Lu
Mar 2, 2016·Proceedings of the National Academy of Sciences of the United States of America·Yohta FukudaEiichi Mizohata
Dec 16, 2016·Journal of Chemical Information and Modeling·Yu-Feng LinChih-Hao Lu

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.