Abstract
Increased expression of cullin 4B (CUL4B) is linked to progression in several cancers. This study aims to explore the effects of CUL4B on bladder cancer (BC) metastasis and epithelial-to-mesenchymal transition (EMT) and potential correlation to the Wnt/β-catenin signaling pathway. We collected BC tissues and adjacent normal tissues from 124 BC patients. Quantitative real-time polymerase chain reaction (qRT-PCR) and western blotting were employed in order to detect the expression of Wnt/β-catenin signaling pathway-related proteins and EMT markers. MTT and Transwell assays were used in order to measure cell proliferation, migration, and invasion. BC 5637 cells were transfected with control, siRNA scramble control (siRNA-NC), si-CUL4B, and CUL4B or Wnt inhibitory factor 1 (WIF-1) overexpression constructs. Levels of CUL4B mRNA and protein were increased in BC tissues in comparison with the adjacent normal tissues. CUL4B expression was negatively correlated with the expression of E-cadherin and positively correlated to the expression of N-cadherin and Vimentin. Compared to the control group, levels of β-catenin, cyclinD1, c-myc, MMP7, and EMT markers were reduced, whereas phosphorylated GSK3β(Ser9) and E-cadherin levels were increa...Continue Reading
References
Feb 1, 2000·International Journal of Cancer. Journal International Du Cancer·M J BussemakersJ A Schalken
Jun 26, 2004·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Sanae NakajimaMasayuki Imamura
Feb 9, 2010·The Journal of Biological Chemistry·Jiejun FuHongmei Wang
Sep 11, 2010·Cancer·Ganesh V RajEugene Frenkel
Sep 30, 2010·International Journal of Oncology·Soichi IwaiYoshiaki Yura
Nov 16, 2010·The Journal of Urology·Justin R GreggDaniel A Barocas
Apr 5, 2011·European Urology·Marko BabjukUNKNOWN European Association of Urology (EAU)
Apr 23, 2011·Cancer Research·Alfred S L ChengJoseph J Y Sung
Aug 19, 2011·JAMA : the Journal of the American Medical Association·Neal D FreedmanChristian C Abnet
Nov 10, 2012·International Journal of Clinical Practice·T R L Griffiths, UNKNOWN Action on Bladder Cancer
Jan 17, 2013·The Journal of Clinical Investigation·Junchao CaiMengfeng Li
Jan 30, 2013·Biochimica Et Biophysica Acta·Fengjuan HeYaoqin Gong
May 8, 2013·Medical Oncology·Tao JiangZhi-hai Peng
Aug 21, 2013·Immunity·Jorg van LoosdregtPaul J Coffer
Oct 16, 2013·The Journal of Experimental Medicine·Aude-Hélène CapiettoRoberta Faccio
Jan 1, 2014·European Urology·J Alfred WitjesAmir Sherif
Sep 6, 2014·Oncology Reports·Zhi ChenLi Chen
Sep 28, 2014·Biochemical and Biophysical Research Communications·Srishti Kotiyal, Susinjan Bhattacharya
Nov 29, 2014·The Journal of Pathology·Jupeng YuanYaoqin Gong
Jan 18, 2015·Pharmacology & Therapeutics·Linna Li, Wenliang Li
Apr 30, 2015·Scientific Reports·Shivani MalikKatrin F Chua
May 7, 2015·Oncotarget·Jupeng YuanYaoqin Gong
May 16, 2015·Journal of Surgical Oncology·Jinfeng WangChuanxin Wang
Jun 6, 2015·Molecular Medicine Reports·Longfeng SunAiping Wang
Oct 10, 2015·Cancer Research·Yanyan QianYaoqin Gong
Feb 27, 2016·Journal of Immunoassay & Immunochemistry·Arfaoui Toumi AmiraS Rammeh
Feb 29, 2016·Journal of Pharmaceutical and Biomedical Analysis·Navneeta BansalAbbas Ali Mahdi
Mar 13, 2016·Clinical Biochemistry·Soon Sun KimJae Youn Cheong
Sep 24, 2016·Oncology Research·Xuguang Wang, Zhe Chen
Citations
Jul 2, 2019·PloS One·Chun-Yu ChenShu-Wha Lin
Aug 15, 2018·International Journal of Molecular Medicine·Qingsong ZhaoYe Liu
Nov 9, 2019·Molecular Medicine Reports·Genquan YueYunfeng Xiao
Apr 11, 2018·Oncotarget·Hui XieZhi Guo
Nov 30, 2018·International Journal of Oncology·Ziling FangXiaojun Xiang
Jul 6, 2020·Journal of Ovarian Research·Peng-Jing DuanLi-Li Xie
Jan 8, 2019·Cell Cycle·Ying LiXin Wang
Mar 19, 2019·Cancer Medicine·Yuanyuan Wang, Dan Yue
Mar 16, 2019·Oncogenesis·Mei QiBo Han
Nov 2, 2019·PloS One·Kazuhiro ShiizakiYasuhiro Mizuta
Dec 20, 2020·Cellular Signalling·Guanlin WuGang Fan
Jan 11, 2021·Cellular Oncology (Dordrecht)·Guang ShanXuedong Wang
Mar 5, 2020·Oncogene·Xiaochen LiuYaoqin Gong
Jun 3, 2021·CNS Neuroscience & Therapeutics·Zong-Qing ZhaoFan Feng
Aug 24, 2021·Frontiers in Molecular Biosciences·Fan FengXueyuan Heng