Cultivable bacteria populations associated with leaves of banana and plantain plants and their antagonistic activity against Mycosphaerella fijiensis.

Microbial Ecology
Isabel CeballosValeska Villegas

Abstract

Mycosphaerella fijiensis is the etiological agent of Black Sigatoka, a fungal disease that affects production of banana and plantain crops in tropical regions. The sizes of cultivable epiphytic and endophytic bacterial populations, aerobic endospore forming bacteria (AEFB), and antagonist bacteria against M. fijiensis isolated from three Musa spp. cultivars from Urabá (Colombia) were studied, in order to find a suitable screening strategy to isolate antagonistic bacteria. Most of the variability found in the epiphytic and endophytic bacterial community sizes among fruit trees was explained by the cultivar differences. We found population sizes ranging from 1.25 × 10(3) to 9.64 × 10(5) CFU/g of fresh leaf and found that 44 % of total cultivable bacteria belong to the AEFB group. We isolated 648 AEFB from three different cultivars and assessed their antagonistic activity against M. fijiensis using the cell-free supernatant obtained from bacterial liquid cultures in three different in vitro assays. Five percent of those bacteria showed higher percent inhibition than the positive control Bacillus subtilis UA321 has (percent inhibition = 84 ± 5) in the screening phase. Therefore, they were selected as antagonistic bacteria against t...Continue Reading

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Citations

Feb 15, 2016·World Journal of Microbiology & Biotechnology·Benjamín Moreno CastilloGraciela Huerta Palacios
Oct 13, 2015·Journal of Microbiological Methods·Lina Escobar-TovarMiguel A Gómez-Lim
May 20, 2015·Fungal Biology·Lina Escobar-TovarMiguel A Gómez-Lim
May 5, 2018·Phytopathology·Charlotte PoeydebatLuc De Lapeyre De Bellaire
Feb 17, 2018·3 Biotech·Mileidy Cruz-MartínYelenys Alvarado-Capó
Aug 17, 2019·Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]·Mileidy Cruz-MartínYelenys Alvarado-Capó

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