Cultivable microbial flora associated with persistent periapical disease and coronal leakage after root canal treatment: a preliminary study

International Endodontic Journal
V AdibKishor Gulabivala

Abstract

To identify the cultivable bacterial flora in root filled teeth with persistent periapical lesions and to locate their distribution within the root canal system using an in vitro sampling protocol. Eight freshly extracted root filled teeth were collected from the Oral and Maxillo-facial Surgery Department, Eastman Dental Hospital. Seven teeth were associated with persistent apical periodontitis and also showed evidence of coronal leakage. Teeth were transferred to an anaerobic chamber immediately after careful extraction and sectioned transversely to give a crown (in all but one case) and two root segments (coronal and apical). Two samples were obtained from each segment, one from dentine and the other from the restoration or gutta-percha (GP) root filling (46 sites in total). The samples were dispersed, serially diluted and cultured on blood agar and fastidious anaerobic agar (with 5% defibrinated horse blood). The primary growth was subcultured to obtain pure isolates, which were identified by routine microbiological techniques and commercial enzyme tests. A total of 252 strains were isolated from all the teeth. Of all the isolates, the most prevalent bacteria were Gram-positive facultative anaerobes 189/252 (75%) with staphy...Continue Reading

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Citations

Mar 17, 2012·International Journal of Oral Science·Qian-Qian WangXiao-Fei Zhu
Nov 26, 2008·Journal of Endodontics·Gaëlle VilletteShimon Friedman
Oct 22, 2008·Journal of Endodontics·José F Siqueira, Isabela N Rôças
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Jan 11, 2007·International Endodontic Journal·C Maniglia-FerreiraF J Souza-Filho
Apr 11, 2006·Australian Endodontic Journal : the Journal of the Australian Society of Endodontology Inc·Marcus T Yan
Feb 1, 2011·International Endodontic Journal·A C M OliveiraM Tanomaru-Filho
Sep 27, 2016·The Bulletin of Tokyo Dental College·Masato NaritaKazuyuki Ishihara
Nov 6, 2007·Brazilian Dental Journal·Cláudio Maniglia-FerreiraFrancisco José de Souza-Filho
Sep 14, 2010·The Journal of Clinical Pediatric Dentistry·Gildardo Ledezma-RasilloAmaury J Pozos-Guillen
Mar 10, 2020·BioMed Research International·Mewan Salahalddin A AlrahmanKawa F Dizaye
May 6, 2019·Dentistry Journal·Mario DioguardiLorenzo Lo Muzio
Sep 23, 2018·Journal of Endodontics·Giovanni MergoniMaddalena Manfredi

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