Cultivated and wild Pleurotus ferulae ethanol extracts inhibit hepatocellular carcinoma cell growth via inducing endoplasmic reticulum stress- and mitochondria-dependent apoptosis

Scientific Reports
Yi YangJinyao Li

Abstract

Pleurotus ferulae is a kind of editable mushroom and has various biological functions such as antitumor, antioxidation and immunoregulation. Wild P. ferulae was successfully domesticated but the antitumor function and mechanisms of cultivated and wild P. ferulae need to be compared and explored. Here, we prepared cultivated and wild P. ferulae ethanol extracts (PFEE-C and PFEE-W) and compared their antitumor effect on hepatocellular carcinoma. Our data showed that PFEE-C and PFEE-W significantly inhibited the growth of H22 and HepG2 cells through induction of apoptosis. PFEE-W exhibited higher antitumor activity than PFEE-C. Both PFEE-C and PFEE-W induced endoplasmic reticulum (ER) stress characterized by the up-regulated levels of phosphorylated JNK, cleaved caspase-12 and HSP70, and mitochondrial dysfunction characterized by the reduction of mitochondrial membrane potential and the release of cytochrome c, which promoted the cleavage of caspase-3, -7, -9 and PARP. Moreover, PFEE-C and PFEE-W significantly increased ROS generation in H22 cells and suppressed H22 cell migration through reducing the levels of matrix metalloproteinase -2 and -9. Further, PFEE-C inhibited H22 tumor growth in mouse model and improved the survival o...Continue Reading

References

Sep 6, 2006·EMBO Reports·Eva SzegezdiAfshin Samali
Jul 25, 2008·The New England Journal of Medicine·Josep M LlovetUNKNOWN SHARP Investigators Study Group
Aug 4, 2009·Journal of Gastroenterology and Hepatology·Jian-Gao FanUNKNOWN Asia-Pacific Working Party for Prevention of Hepatocellular Carcinoma
Mar 3, 2010·Journal of Medicinal Food·Fatih KalyoncuA Usame Tamer
Aug 5, 2010·Nature Reviews. Molecular Cell Biology·Stephen W G Tait, Douglas R Green
Aug 6, 2010·Cancer Letters·Min Li-Weber
Mar 3, 2011·Nature Cell Biology·Ira Tabas, David Ron
May 12, 2012·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Sasan RoayaieVincenzo Mazzaferro
Aug 25, 2012·Current Biology : CB·Stewart Siyan Cao, Randal J Kaufman
Nov 24, 2012·Nature Reviews. Molecular Cell Biology·Lorenzo GalluzziGuido Kroemer
Jan 20, 2016·Evidence-based Complementary and Alternative Medicine : ECAM·Mallappa Kumara SwamyMohd Sayeed Akhtar
Jan 24, 2016·Apoptosis : an International Journal on Programmed Cell Death·Qiang ZhangXiao Yuan
Jan 26, 2016·CA: a Cancer Journal for Clinicians·Wanqing ChenJie He
Apr 11, 2017·Cancer Chemotherapy and Pharmacology·Qiang WangHui Ding
Jul 1, 2017·JAMA Oncology·Christina Fitzmaurice, Mohsen Naghavi

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Methods Mentioned

BETA
flow cytometry
fluorescence microscopy
fluorescence

Software Mentioned

Image J
Prism

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