Cultivation and Genomic Analysis of "Candidatus Nitrosocaldus islandicus," an Obligately Thermophilic, Ammonia-Oxidizing Thaumarchaeon from a Hot Spring Biofilm in Graendalur Valley, Iceland

Frontiers in Microbiology
Anne DaebelerMichael Wagner

Abstract

Ammonia-oxidizing archaea (AOA) within the phylum Thaumarchaeota are the only known aerobic ammonia oxidizers in geothermal environments. Although molecular data indicate the presence of phylogenetically diverse AOA from the Nitrosocaldus clade, group 1.1b and group 1.1a Thaumarchaeota in terrestrial high-temperature habitats, only one enrichment culture of an AOA thriving above 50°C has been reported and functionally analyzed. In this study, we physiologically and genomically characterized a newly discovered thaumarchaeon from the deep-branching Nitrosocaldaceae family of which we have obtained a high (∼85%) enrichment from biofilm of an Icelandic hot spring (73°C). This AOA, which we provisionally refer to as "Candidatus Nitrosocaldus islandicus," is an obligately thermophilic, aerobic chemolithoautotrophic ammonia oxidizer, which stoichiometrically converts ammonia to nitrite at temperatures between 50 and 70°C. "Ca. N. islandicus" encodes the expected repertoire of enzymes proposed to be required for archaeal ammonia oxidation, but unexpectedly lacks a nirK gene and also possesses no identifiable other enzyme for nitric oxide (NO) generation. Nevertheless, ammonia oxidation by this AOA appears to be NO-dependent as "Ca. N. ...Continue Reading

References

Apr 1, 1977·Journal of Bacteriology·D H Gelfand, R A Steinberg
Jun 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K MaM W Adams
Feb 1, 1996·Applied and Environmental Microbiology·J ZhouJ M Tiedje
Nov 25, 1998·Proceedings of the National Academy of Sciences of the United States of America·I K CannY Ishino
Dec 11, 1999·Extremophiles : Life Under Extreme Conditions·Q A AlmatawahD A Cowan
Feb 17, 2001·Nitric Oxide : Biology and Chemistry·K M MirandaD A Wink
Sep 6, 2001·Genome Biology·H C Pace, C Brenner
Jun 1, 1995·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·M Schulte, E Shock
Jun 1, 2002·Science·Errol C FriedbergMiroslav Radman
Sep 14, 2002·Origins of Life and Evolution of the Biosphere : the Journal of the International Society for the Study of the Origin of Life·Shin MiyakawaStanley L Miller
Jul 31, 1959·Science·S L MILLER, H C UREY
Feb 27, 2004·Nucleic Acids Research·Wolfgang LudwigKarl-Heinz Schleifer
Jul 3, 2004·Journal of Bacteriology·Haruyuki AtomiTadayuki Imanaka
Sep 15, 2005·Journal of Bacteriology·Konstantinos T Konstantinidis, James M Tiedje
Sep 24, 2005·Nature·Martin KönnekeDavid A Stahl
Sep 28, 2005·Proceedings of the National Academy of Sciences of the United States of America·Christopher A FrancisBrian B Oakley
May 9, 2006·Bioinformatics·Marc A Suchard, Benjamin D Redelings
Aug 18, 2006·Nature·S LeiningerC Schleper
Nov 23, 2006·Proceedings of the National Academy of Sciences of the United States of America·Steven J HallamEdward F DeLong
May 8, 2007·Nucleic Acids Research·Lukas KällErik L L Sonnhammer
Jan 8, 2008·International Journal of Systematic and Evolutionary Microbiology·E V LebedevaE Spieck
Jan 22, 2008·Environmental Microbiology·José R de la TorreDavid A Stahl
Feb 6, 2008·Proceedings of the National Academy of Sciences of the United States of America·Roland HatzenpichlerMichael Wagner
Feb 16, 2008·Nature Reviews. Microbiology·Céline Brochier-ArmanetPatrick Forterre
Mar 22, 2008·FEMS Microbiology Ecology·Laila J ReigstadChrista Schleper
Aug 5, 2008·Applied and Environmental Microbiology·Chuanlun L ZhangBrian P Hedlund
Apr 28, 2009·Applied and Environmental Microbiology·Shufang WangFengping Wang
May 21, 2009·FEMS Microbiology Reviews·Tuba H ErguderWilly Verstraete
Jun 19, 2009·Bioinformatics·Nicolas LartillotSamuel Blanquart
Jun 27, 2009·The Journal of Microbiology·Michelle Heine, Sathees B C Chandra
Feb 6, 2010·Environmental Microbiology·Rita BartossekChrista Schleper
Mar 10, 2010·BMC Bioinformatics·Doug HyattLoren J Hauser
Mar 13, 2010·PloS One·Morgan N PriceAdam P Arkin

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Citations

Feb 19, 2019·Environmental Microbiology·Mart KrupovicEugene V Koonin
Oct 19, 2019·The ISME Journal·Eva SpieckJennifer Hüpeden
Jun 7, 2018·FEMS Microbiology Ecology·Michiel H In 't ZandtMike Sm Jetten
Jul 1, 2020·International Journal of Systematic and Evolutionary Microbiology·Aharon OrenMartha E Trujillo
Jun 11, 2020·BMC Biology·Eugene V KooninYoshizumi Ishino
Sep 6, 2020·The ISME Journal·Nicholas J ReichartRoland Hatzenpichler
Apr 25, 2019·Nature Communications·K Dimitri KitsHolger Daims
Sep 29, 2020·Frontiers in Microbiology·Dayu ZouMeng Li
Jul 22, 2018·Nature Communications·Zheng-Shuang HuaWen-Jun Li
Sep 26, 2020·Nucleic Acids Research·Darius KazlauskasČeslovas Venclovas
Oct 18, 2020·The ISME Journal·Anna J MuellerHolger Daims
Nov 1, 2020·Nature Communications·Paul O SheridanCécile Gubry-Rangin
Jan 28, 2021·FEMS Microbiology Ecology·Rebecca C MuellerBrent M Peyton
Dec 14, 2018·Emerging Topics in Life Sciences·Cécile Gubry-RanginJames I Prosser

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Datasets Mentioned

BETA
CP024014
PRJEB24462

Methods Mentioned

BETA
PCR
amplicon sequencing

Software Mentioned

MicroScope
CheckM
Unicycler
Phobius
Bali
ARB aligner
Phyre2
blastp
IMNGS
hmmsearch

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