Cultivation and partial characterization of bovine astrovirus

Veterinary Microbiology
D AroonprasertG N Woode

Abstract

Bovine astrovirus serotype 2 (US2) was adapted to primary neonatal kidney cell (NBK) cultures by the addition of 50 micrograms ml-1 of trypsin in the medium. Infectious virus was released from the cells within 7 days post-infection in early passages and within 3 days in later passages. In the absence of trypsin, neither passage of infected cells nor release of infectious virus occurred. The virus was shown to be similar to the fecal astrovirus by a neutralization test and by ultrastructural studies of infected cells. Primary embryo bovine kidney (EBK) and NBK cell cultures supported infection with both fecal and tissue culture adapted (TCA) astrovirus. The time-related development of infection, as studied by immunofluorescence, was similar for both fecal and TCA astrovirus and for both cell culture types. The first indication of viral infection and expression of viral antigens occurred at 7 h post-infection and was characterized by the appearance of a diffuse faint immunofluorescence (IF) of the cytoplasm. Soon after, two or three brilliant IF granules were observed in the nucleus, which appeared to involve the nucleoli. Subsequently, dense granular IF was seen in the perinuclear region of the cytoplasm, which later extended to...Continue Reading

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Sep 1, 1990·Baillière's Clinical Gastroenterology·W D Cubitt
Nov 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·B JiangR I Glass
Oct 25, 2002·Avian Pathology : Journal of the W.V.P.A·Matthew D Koci, Stacey Schultz-Cherry
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Jul 3, 2021·Viruses·Nicole Wildi, Torsten Seuberlich

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