Cultivation-independent methods reveal differences among bacterial gut microbiota in triatomine vectors of Chagas disease.

PLoS Neglected Tropical Diseases
Fabio Faria da MotaPatrícia Azambuja

Abstract

Chagas disease is a trypanosomiasis whose agent is the protozoan parasite Trypanosoma cruzi, which is transmitted to humans by hematophagous bugs known as triatomines. Even though insecticide treatments allow effective control of these bugs in most Latin American countries where Chagas disease is endemic, the disease still affects a large proportion of the population of South America. The features of the disease in humans have been extensively studied, and the genome of the parasite has been sequenced, but no effective drug is yet available to treat Chagas disease. The digestive tract of the insect vectors in which T. cruzi develops has been much less well investigated than blood from its human hosts and constitutes a dynamic environment with very different conditions. Thus, we investigated the composition of the predominant bacterial species of the microbiota in insect vectors from Rhodnius, Triatoma, Panstrongylus and Dipetalogaster genera. Microbiota of triatomine guts were investigated using cultivation-independent methods, i.e., phylogenetic analysis of 16s rDNA using denaturing gradient gel electrophoresis (DGGE) and cloned-based sequencing. The Chao index showed that the diversity of bacterial species in triatomine guts ...Continue Reading

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Datasets Mentioned

BETA
FR729479.1
EF530551.1
AJ292347.1
DQ115537.1
FJ152101.1

Methods Mentioned

BETA
dissection
PCR
electrophoresis

Software Mentioned

Phred
CAP3
BLASTn
MUSCLE
Mallard
MOTHUR OTU
PHYLIP
DNADIST
MOTHUR

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