Cultivation of Bacteria From Aplysina aerophoba : Effects of Oxygen and Nutrient Gradients

Frontiers in Microbiology
Johanna GutlebenDetmer Sipkema

Abstract

Sponge-associated bacteria possess biotechnologically interesting properties but as yet have largely evaded cultivation. Thus, "omics"-based information on the ecology and functional potential of sponge symbionts is awaiting its integration into the design of innovative cultivation approaches. To cultivate bacteria derived from the marine sponge Aplysina aerophoba, nine novel media formulations were created based on the predicted genomic potential of the prevalent sponge symbiont lineage Poribacteria. In addition, to maintain potential microbial metabolic interactions in vitro, a Liquid-Solid cultivation approach and a Winogradsky-column approach were applied. The vast majority of microorganisms in the inoculum appeared viable after cryopreservation of sponge specimen as determined by selective propidium monoazide DNA modification of membrane-compromised cells, however, only 2% of the initial prokaryotic diversity could be recovered through cultivation. In total, 256 OTUs encompassing seven prokaryotic phyla were cultivated. The diversity of the cultivated community was influenced by the addition of the antibiotic aeroplysinin-1 as well as by medium dilution, rather than carbon source. Furthermore, the Winogradsky-column approa...Continue Reading

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Datasets Mentioned

BETA
PRJEB31820
1
CP036425.1

Methods Mentioned

BETA
amplicon sequencing
PMA
PCR
scraping
electrophoresis

Software Mentioned

vegan
Adobe Photoshop
Venny
R
phyloseq
picante
Tax
R stats
DESeq2
NG

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