PMID: 2505214Jun 1, 1989Paper

Cultivation of Blastocrithidia triatomae (Trypanosomatidae) on a cell line of its host Triatoma infestans (Reduviidae)

Parasitology
D ReduthM Pudney

Abstract

Blastocrithidia triatomae parasitizes vectors of Chagas' disease and is very difficult to cultivate in conventional media. However, co-cultivation with a cell line of its host Triatoma infestans (TI-32; in Schneider's Drosophila medium supplemented with 20% foetal calf serum and 10% tryptose phosphate broth) led to vigorous growth at 24 or 28 degrees C without an adaptation phase. More than 60 primary cultures were initiated successfully without any failures. Subcultures could be started immediately at weekly intervals. The doubling time was similar in both the primary cultures (41 h) and the 33rd subculture (39 h). The importance of the reduviid cells for B. triatomae became clear after removal of the insect cells, when multiplication of epimastigotes stopped and mainly cysts were formed. Cysts produced in vitro were infective for reduviids. Scanning electron microscopy showed that B. triatomae attached to the host cells, inserted its flagellum into them and destroyed them.

References

Jan 1, 1988·Transactions of the Royal Society of Tropical Medicine and Hygiene·G A Schaub
Feb 1, 1988·Veterinary Parasitology·G C Coles
Aug 1, 1987·Annals of Tropical Medicine and Parasitology·S MaraghiD H Molyneux
May 1, 1987·Acta Neurologica Scandinavica·K Jensen
May 1, 1985·The American Journal of Tropical Medicine and Hygiene·D L Sacks, P V Perkins
Aug 1, 1971·The Journal of protozoology·J A CerisolaC E Del Prado
Mar 30, 1984·Science·D L Sacks, P V Perkins
Jan 1, 1981·Transactions of the Royal Society of Tropical Medicine and Hygiene·G A Schaub, M Pretsch

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Citations

Jan 1, 1990·Parasitology Today·G A SchaubM Pudney
Mar 31, 2009·International Journal of Systematic and Evolutionary Microbiology·Vyacheslav Y YurchenkoDmitri A Maslov

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