Cultivation of Drought-Tolerant and Insect-Resistant Rice Affects Soil Bacterial, but Not Fungal, Abundances and Community Structures

Frontiers in Microbiology
Peng LiXueming Tang

Abstract

The impacts of rice varieties with stacked drought tolerance and insect resistance on soil microbiomes are poorly understood. Hence, the objective of this study was to investigate the effects resulting from the cultivation of the drought-tolerant and insect-resistant rice cultivar, Hanhui3T, on soil physical-chemical properties, and bacterial and fungal community composition. Soil samples of Hanhui3T and conventional rice varieties (Hanhui3 and Zhonghua11) were collected in triplicate at the booting stage, and bacterial and fungal population sizes and community structures were assessed using qPCR and Illumina MiSeq sequencing, respectively. The Bt protein concentration of Hanhui3T was significantly higher than that of Hanhui3 and Zhonghua11, while the pH of Hanhui3T was significantly lower. Bacterial population sizes and community composition were significantly different between Hanhui3T and Hanhui3 (or Zhonghua11), while no similar effects were observed for fungal communities. These differences suggest that the effect of Hanhui3T cultivation on bacterial community composition is stronger than the effect on fungal communities. Moreover, bacterial abundance was positively correlated to soil pH, while bacterial community structur...Continue Reading

References

Dec 11, 1999·Nature·D SaxenaG Stotzky
Oct 31, 2000·Applied and Environmental Microbiology·M T SuzukiE F DeLong
Sep 4, 2004·Environmental Microbiology·Nabla KennedyNicholas Clipson
Jan 14, 2005·Critical Reviews in Microbiology·E SomersM Srinivasan
May 17, 2005·Environmental Microbiology·Nabla KennedyNicholas Clipson
Nov 14, 2008·The ISME Journal·Kristen M DeAngelisMary K Firestone
Apr 13, 2010·Nature Methods·J Gregory CaporasoRob Knight
May 7, 2010·The ISME Journal·Johannes RouskNoah Fierer
Jun 28, 2011·Bioinformatics·Robert C EdgarRob Knight
Aug 12, 2014·Applied and Environmental Microbiology·Simone Raposo CottaJan Dirk van Elsas
Mar 3, 2015·Research in Microbiology·Alessandra TurriniManuela Giovannetti
May 15, 2015·Applied Microbiology and Biotechnology·Petra ValldorChristoph C Tebbe
Apr 20, 2016·Journal of Microbiology and Biotechnology·Soo-In SohnHyun-Suk Cho

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Datasets Mentioned

BETA
SRP130072

Methods Mentioned

BETA
genetic modification
transgenic
PCR
enzyme-linked immunosorbent assays
ELISA
PCRs

Software Mentioned

RDP Classifier
vegan
R
QIIME
Cluster Database at High Identity with Tolerance ( CD - HIT )
QIIME Pipeline
SPSS
mothur

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