Cultivation of endothelial progenitor cells on fibrin matrix and layering on dacron/polytetrafluoroethylene vascular grafts

Artificial Organs
Perumcherry Raman Sreerekha, Lissy K Krishnan

Abstract

Completely biological tissue-engineered vascular graft is an upcoming substitute for damaged blood vessel, but its clinical use is currently limited due to poor mechanical strength. Therefore, at present, polymeric small-diameter vascular grafts lined with endothelial cells (ECs) to reduce graft thrombosis may be a more viable option. Successful construction of EC-seeded artificial grafts faces some challenges such as (i) retention of endothelial lining; and (ii) availability of differentiated autologous cells. Biomaterial surfaces that are modified by depositing extracellular matrix (ECM) components may stabilize cells in the lumen against forces of blood flow. Adult stem cells such as endothelial progenitor cells (EPCs) circulate in the blood and they usually attach to the exposed matrix at the injured blood vessel site. Depending on the signaling capabilities of ECM, cells may differentiate into ECs,, and if a similar composition of the matrix is provided in vitro, EPCs isolated from blood might get differentiated and thus autologous cells for tissue engineering may be obtained. In this in vitro study, ECM scaffold consisting of biomolecules such as fibrin, fibronectin, and gelatin along with growth factors is found to have ...Continue Reading

References

Nov 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·T MaciagR Forand
Jul 1, 1992·Journal of Molecular and Cellular Cardiology·K ShivakumarM S Valiathan
Feb 1, 1996·The Journal of Surgical Research·C GosselinH P Greisler
Jul 1, 1997·Pharmaceutical Research·R Langer
Jan 15, 1998·Plastic and Reconstructive Surgery·E F RitterB Klitzman
Apr 25, 2000·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Q YeM Turina
May 18, 2000·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·Q YeM Turina
Apr 18, 2001·European Journal of Cardio-thoracic Surgery : Official Journal of the European Association for Cardio-thoracic Surgery·S JockenhoevelM Turina
Dec 3, 2002·Tissue Engineering·T R Santhosh Kumar, Lissy K Krishnan
Sep 7, 2004·Journal of Materials Science. Materials in Medicine·T R S Kumar, L K Krishnan

❮ Previous
Next ❯

Citations

Mar 7, 2007·Medical & Biological Engineering & Computing·Sandip SarkarAlexander M Seifalian
Feb 1, 2007·Molecular Therapy : the Journal of the American Society of Gene Therapy·Chou Chai, Kam W Leong
Sep 10, 2011·Tissue Engineering. Part C, Methods·Sreerekha Raman PerumcherryRajan Afeesh
Mar 23, 2013·Cardiovascular Research·Cheng ZhangQingbo Xu
Sep 20, 2008·Journal of the Royal Society, Interface·Paul A JanmeyJohn W Weisel
Mar 9, 2007·Artificial Organs·Paul S Malchesky
Apr 15, 2010·Cardiovascular Research·Juliane LadhoffMartina Seifert
Feb 25, 2014·Journal of Artificial Organs : the Official Journal of the Japanese Society for Artificial Organs·Shunsuke SakakibaraHiroto Terashi
Sep 6, 2013·Tissue Engineering. Part B, Reviews·Jeremy J Glynn, Monica T Hinds
Dec 25, 2008·Expert Review of Medical Devices·Dong-Choon SinXigeng Miao
Jan 16, 2013·Journal of Tissue Engineering and Regenerative Medicine·Stephen McIlhennyPaul DiMuzio
Mar 14, 2009·Journal of Tissue Engineering and Regenerative Medicine·Aditee Kurane, Naren Vyavahare
Jun 1, 2013·Journal of Biomedical Materials Research. Part a·Elena FilováLucie Bačáková
Feb 7, 2012·Biomaterials·Jeffrey T Krawiec, David A Vorp
Sep 24, 2013·Biomaterials·Murielle RémyLaurence Bordenave
Nov 27, 2007·Journal of Tissue Engineering and Regenerative Medicine·Divya PankajakshanLissy K Krishnan
Nov 23, 2020·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·S R AravindLissy K Krishnan
Aug 13, 2018·ACS Biomaterials Science & Engineering·Juan Felipe Diaz QuirozMariah S Hahn

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adult Stem Cells

Adult stem cells reside in unique niches that provide vital cues for their survival, self-renewal, and differentiation. They hold great promise for use in tissue repair and regeneration as a novel therapeutic strategies. Here is the latest research.

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.