Cultivation of hepatitis C virus in primary hepatocyte culture from patients with chronic hepatitis C results in release of high titre infectious virus

The Journal of General Virology
T ItoKotaro Yasui

Abstract

To investigate the viral replication cycle and genomic heterogeneity of hepatitis C virus (HCV), we established an HCV cultivation system by using a primary hepatocyte culture from patients with chronic hepatitis C. Liver tissue was obtained by needle biopsy or surgery, then hepatocytes were isolated by collagenase digestion. After several weeks, we determined the HCV RNA titre of the cultured cells and supernatant by a competitive polymerase chain reaction (PCR) method. A significant amount of HCV RNA was observed in the cells and supernatant during cultivation. Negative-strand RNA, regarded as a marker of viral replication, could be detected by a strand-specific reverse transcription PCR method and the HCV core protein could be detected by immunofluorescence microscopy. Many HCV particles released into the supernatant were infectious. In addition, we compared the nucleotide sequences in the E2/NS1 region of pre- and post-cultivation hepatocytes for 8 weeks. At the beginning of the culture period, three major HCV types containing two subtypes were isolated. Following cultivation, the same types were isolated from the cultured hepatocytes in the same ratio as prior to cultivation. We could detect the same clones in this patient...Continue Reading

Citations

Apr 1, 1997·Reviews in Medical Virology·F Radecke, M A Billeter
Feb 14, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·H AizakiT Miyamura
Jul 10, 1998·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·D MoradpourH E Blum
Sep 1, 2001·Antiviral Research·R Bartenschlager, V Lohmann
Sep 17, 1999·Journal of Hepatology·A ManzinM Clementi
May 19, 2001·Microbes and Infection·A SteffanF Stoll-Keller
May 9, 2000·Journal of Virology·S TakikawaT Miyamura
Apr 7, 2011·Genetic Vaccines and Therapy·Usman Ali AshfaqSheikh Riazuddin
Dec 7, 2013·World Journal of Gastroenterology : WJG·Yue Wang
Jun 21, 2011·Annual Review of Microbiology·Catherine L Murray, Charles M Rice
Mar 16, 2013·Gastroenterología y hepatología·George KoutsoudakisSofía Pérez-Del-Pulgar
Apr 27, 1999·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M A LoriotC Bréchot
Mar 9, 2010·Gastroenterología y hepatología·Reyes Aparcero LópezManuel Romero-Gómez
Jun 27, 2009·Pharmacology & Therapeutics·Claire GondeauPatrick Maurel
Dec 14, 2006·Clinics in Liver Disease·Chen Liu
Mar 24, 2009·Liver International : Official Journal of the International Association for the Study of the Liver·Valérie-Ann RaymondMarc Bilodeau
May 6, 2004·Experimental and Molecular Pathology·Ramesh PrabhuSrikanta Dash
Nov 9, 2011·Infection, Genetics and Evolution : Journal of Molecular Epidemiology and Evolutionary Genetics in Infectious Diseases·Huma TariqIshtiaq Qadri
Apr 1, 2011·Saudi Journal of Biological Sciences·Zaki Monawar Eisa
Aug 3, 2001·Biologicals : Journal of the International Association of Biological Standardization·D ForcićR Mazuran
May 18, 2016·Saudi Journal of Gastroenterology : Official Journal of the Saudi Gastroenterology Association·Medhat K ShierMohammad M Al-Qattan
Aug 4, 1999·Antiviral Chemistry & Chemotherapy·R F SchinaziS Dagan
Apr 25, 1997·The Journal of Biological Chemistry·R B RayR Ray
Jun 11, 2005·World Journal of Gastroenterology : WJG·Min LiuShu-Mei Lin
Jun 10, 2004·World Journal of Gastroenterology : WJG·Min LiuQian Yang
Dec 21, 2006·Journal of Medical Virology·Matthieu CarrièrePhilippe Podevin
Nov 9, 2005·Microscopy Research and Technique·Srikanta DashRobert Garry
Mar 3, 2004·The Journal of Biological Chemistry·Tomoko DateTakaji Wakita
Jun 22, 2000·The Journal of General Virology·R Bartenschlager, V Lohmann
Aug 16, 2001·Journal of Medical Virology·C Caussin-SchwemlingF Stoll-Keller

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