PMID: 7208105Feb 1, 1981

Cultivation of infective forms of Trypanosoma congolense from trypanosomes in the proboscis of Glossina morsitans

Parasitology
M A GrayA G Luckins

Abstract

Two stocks of Trypanosoma congolense were established in culture at 28 degrees C using trypanosomes from the proboscides of infective Glossina morsitans. Successful primary cultures were initiated by placing an infected tsetse proboscis beside a bovine dermal collagen explant in Eagle's minimum essential medium supplemented with foetal calf serum. The trypanosomes multiplied rapidly in the medium and also gradually formed an adherent layer o the plastic surface of the culture vessel. Three primary cultures produced organisms infective for mice from 14, 20 and 35 days after initiation and thereafter continuously until days 76, 76 and 52 when they were discarded. Four attempts to initiate infective cultures using infected tsetse proboscides in medium without dermal explants were unsuccessful. When trypanosomes from primary cultures were placed in culture medium with proboscides from uninfected tsetse flies, the parasites multiplied, formed an adherent layer in the culture flasks and were seen in the proboscides within 24 h. A line of 1 stock was serially sub-passaged in this way 4 times during a period of 215 days. Infectivity titrations in mice indicated that primary and sub-passaged cultures each contained similar numbers of in...Continue Reading

References

Feb 1, 1979·The Journal of protozoology·K L Banks
Aug 1, 1979·The Journal of protozoology·I Cunningham, A M Taylor
Jan 1, 1979·Transactions of the Royal Society of Tropical Medicine and Hygiene·M A GrayA R Gray
Apr 13, 1978·Nature·A G Luckins, A R Gray
Feb 1, 1973·Experimental Parasitology·I Cunningham
Jan 1, 1967·Advances in Enzyme Regulation·B C Johnson, H F Sassoon
Dec 1, 1959·Annals of Tropical Medicine and Parasitology·W TRAGER
Nov 1, 1962·The Journal of protozoology·L E STEPHEN
Dec 1, 1963·Experimental Parasitology·W H LUMSDENP J WALKER

Citations

Jan 1, 1984·Transactions of the Royal Society of Tropical Medicine and Hygiene·P Dukes
Jul 1, 1983·Proceedings of the National Academy of Sciences of the United States of America·M L MuhichA M Simpson
Nov 12, 1984·Nucleic Acids Research·J Shlomai, A Zadok
Jun 9, 2012·Parasites & Vectors·Lori PeacockWendy Gibson
Aug 1, 1985·Veterinary Parasitology·M Murray, S J Black
Feb 1, 1996·Tropical Animal Health and Production·R BoidA G Luckins
Mar 1, 1990·The Journal of protozoology·M A AbdallahF M al-Yaman
Jun 3, 2004·Experimental Parasitology·Majed H Wakid, Paul A Bates
May 1, 1984·The Journal of protozoology·E ReinwaldH J Risse
Nov 22, 2013·PloS One·Richard John WheelerKeith Gull
May 1, 1988·Journal of Ultrastructure and Molecular Structure Research·U Frevert, E Reinwald
Nov 13, 1984·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·M J Turner
Aug 1, 1987·Annals of Tropical Medicine and Parasitology·S MaraghiD H Molyneux
Jun 1, 1984·Annals of Tropical Medicine and Parasitology·H Hirumi, K Hirumi
Apr 1, 1990·Annals of Tropical Medicine and Parasitology·J K StilesD H Molyneux
Feb 2, 2019·Current Protocols in Microbiology·Chris KayWendy Gibson

Related Concepts

Zyderm
Melting
Vestibule of Mouth
Medical Parasitology
Nannomonas
Glossina
Culture Techniques

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