Cultivation of mammalian cells in heat-sealable pouches that are permeable to carbon dioxide

Experimental Cell Research
R J KlebeG Zardeneta

Abstract

Petri plates, 96-well plates, and other unsealed culture vessels are the chief cause of air-borne contamination of cell cultures. In this study, heat-sealable plastic pouches that are permeable to CO2 and other gases are used as a means to avoid contamination. Several applications of heat-sealable pouches are described. First, petri plates can simply be sealed in a wide variety of plastic films that are permeable to CO2. Second, a few CO2-permeable plastic films can be used directly as substrata for mammalian cell growth and can also be cut with a simple hole punch for the isolation of clones. Third, one can grow cells in chemically sterilized carbonic acid solutions and thereby avoid the use of a CO2 incubator entirely.

References

Jul 1, 1975·Journal of Clinical Microbiology·C F Amstein, P A Hartman
Apr 1, 1977·Science·W W NicholsS A Dwight
Jan 1, 1989·Journal of Biomaterials Applications·D J Dempsey, R R Thirucote
Jan 1, 1989·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·J P Freyer, P L Schor
Sep 1, 1985·Journal of Biomedical Materials Research·K L Bentley, R J Klebe
Jan 1, 1985·In Vitro Cellular & Developmental Biology : Journal of the Tissue Culture Association·R T PrzygodaA Tummey
Nov 1, 1973·Journal of the National Cancer Institute·D J GiardW P Parks
Apr 1, 1974·Experimental Cell Research·G R Martin, H Rubin
Nov 1, 1983·The Journal of Cell Biology·A S CurtisF Lawrie
Mar 11, 1983·Science·R T Taggart, I M Samloff
Mar 1, 1984·Environmental Health Perspectives·M G Gabridge, M F Gladd
Dec 1, 1981·Journal of Cellular Physiology·R J KlebeR C Schoen

❮ Previous
Next ❯

Citations


❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.