Cultural conditions on the production of extracellular enzymes by Trichoderma isolates from tobacco rhizosphere

Brazilian Journal of Microbiology : [publication of the Brazilian Society for Microbiology]
K L N Mallikharjuna RaoH Ravisankar

Abstract

Twelve isolates of Trichoderma spp. isolated from tobacco rhizosphere were evaluated for their ability to produce chitinase and β-1,3-glucanase extracellular hydrolytic enzymes. Isolates ThJt1 and TvHt2, out of 12 isolates, produced maximum activities of chitinase and β-1,3-glucanase, respectively. In vitro production of chitinase and β-1,3-glucanase by isolates ThJt1 and TvHt2 was tested under different cultural conditions. The enzyme activities were significantly influenced by acidic pH and the optimum temperature was 30°C. The chitin and cell walls of Sclerotium rolfsii, as carbon sources, supported the maximum and significantly higher chitinase activity by both isolates. The chitinase activity of isolate ThJt1 was suppressed significantly by fructose (80.28%), followed by glucose (77.42%), whereas the β-1,3-glucanase activity of ThJt1 and both enzymes of isolate TvHt2 were significantly suppressed by fructose, followed by sucrose. Ammonium nitrate as nitrogen source supported the maximum activity of chitinase in both isolates, whereas urea was a poor nitrogen source. Production of both enzymes by the isolates was significantly influenced by the cultural conditions. Thus, the isolates ThJt1 and TvHt2 showed higher levels of ...Continue Reading

References

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Mar 24, 2004·Nature Reviews. Microbiology·Gary E HarmanMatteo Lorito
May 12, 2010·Annual Review of Phytopathology·Matteo LoritoEnrique Monte
Dec 3, 2013·Indian Journal of Microbiology·Mala MukherjeeSusanne Zeilinger

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Citations

Jun 30, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Qiulan WuXiangru Liao
Jul 3, 2021·Journal of Fungi·Lucile PellanSabine Schorr-Galindo
Apr 3, 2018·Journal of Natural Products·Cristina Pinedo-RivillaJosefina Aleu

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BETA
chemical treatment

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SAS

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