Cultural, histochemical, and immunohistochemical detection of pathogenic Leptospira species in the kidneys of cattle slaughtered in two abattoirs in Southwest Nigeria

Journal of Immunoassay & Immunochemistry
Olusola Lawrence AjayiOluwawemimo Oluseun Adebowale

Abstract

The impact of leptospirosis on food animals and humans has not been fully elucidated. This study was designed to determine the prevalence of pathogenic Leptospira organism in renal tissues of slaughtered cattle from two major abattoirs in southwestern Nigeria. One hundred and seventy (170) kidney samples of different breeds, ages, and sexes were collected and examined using cultural isolation, microscopic agglutination test (MAT), Warthin silver (WSs) impregnation and immunohistochemistry (IH). Chi-square test was used with a confidence level set at 0.05 to ascertain associations between positive cases and sex, age, and breed. One hundred and thirty (76.5%) of the kidney samples were culturally positive while 83.3% (25/31), 41.6% (37/89), and 35% (7/20) were MAT, WSss, and immunohistochemically positive, respectively. Interstitial nephritis (87%, 94/108) and tubular nephrosis (77.8%, 84/108) were the most prominent and consistent microscopic lesions observed.Serovars Hardjoprajitno (29%) and Bratislava (22.6%) were the most prevalent serovars. To the best of our knowledge, studies on determination of pathogenic Leptospira serovars using cultural isolation, MAT, WSSs, and IH from kidneys of naturally infected cattle in Nigeria a...Continue Reading

References

Sep 1, 1989·Veterinary Pathology·E ScanzianiG Mandelli
Mar 1, 1988·Australian Veterinary Journal·N W SkilbeckM Dohnt
Jul 1, 1967·Journal of Bacteriology·R C Johnson, V G Harris
Nov 1, 1994·The Veterinary Clinics of North America. Food Animal Practice·W A Ellis
Feb 24, 2001·The Veterinary Clinics of North America. Equine Practice·J M Donahue, N M Williams
Apr 9, 2001·Clinical Microbiology Reviews·P N Levett
Oct 16, 2001·Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine·Z Yener, H Keles
Jul 9, 2002·Drug Development and Industrial Pharmacy·S A Sande, K Dyrstad
Jan 1, 1965·American Industrial Hygiene Association Journal·E C RILEYW L SUTTON
May 24, 2008·International Journal of Cosmetic Science·A M MaccioniS Dessì
Jul 16, 2013·Biologicals : Journal of the International Association of Biological Standardization·Marta A Guerra
Sep 18, 2015·PLoS Neglected Tropical Diseases·Federico CostaAlbert I Ko
Apr 12, 2017·The Onderstepoort Journal of Veterinary Research·Olusola L AjayiOluwabusola G Ojebiyi
Dec 1, 1989·Revue Scientifique Et Technique·A O EzehA A Adesiyun

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